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Information Journal Paper

Title

iTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury

Pages

  832-841

Abstract

 Objective(s): Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. However, there has been no report yet on kinds of Proteomics changes that occur in Schwann cells before and after Peripheral nerve injury. Materials and Methods: Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar rat sciatic nerves. After immunofluorescence identification, we identified differentially expressed Proteins in the ASCs and NSCs using isobaric tags for relative and absolute quantitation (iTRAQ) combined with high-resolution Orbitrap liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). In addition, all the differentially expressed Proteins were analyzed by Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. Finally, several differentially expressed Proteins were selected for Western blot verification. Results: A total of 122 differentially expressed Proteins in ASCs and NSCs were screened. GO analysis suggested that these different Proteins are likely to accumulate in the cytoplasm and are associated with single-multicellular organism processes. The KEGG pathway analysis suggested that Proteins related to purine metabolism were significantly enriched. The expression of Transmembrane glycoprotein NMB (GPNMB), Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), and other Proteins were consistent with the Proteomics data obtained by Western blot analysis. Conclusion: GPNMB, ENPP3, GFPT2, and other Proteins may play an important role in the repair of Peripheral nerve injury. This study may provide new insights into changes in SCs after Peripheral nerve injury.

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    APA: Copy

    Shi, Gui Dong, Cheng, Xin, Zhou, Xian Hu, Fan, Bao You, Ren, Yi Ming, LIN, WEI, Zhang, Xiao Lei, Liu, Shen, Hao, Yan, Wei, Zhi Jian, & Feng, Shi Qing. (2018). iTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(8), 832-841. SID. https://sid.ir/paper/725725/en

    Vancouver: Copy

    Shi Gui Dong, Cheng Xin, Zhou Xian Hu, Fan Bao You, Ren Yi Ming, LIN WEI, Zhang Xiao Lei, Liu Shen, Hao Yan, Wei Zhi Jian, Feng Shi Qing. iTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(8):832-841. Available from: https://sid.ir/paper/725725/en

    IEEE: Copy

    Gui Dong Shi, Xin Cheng, Xian Hu Zhou, Bao You Fan, Yi Ming Ren, WEI LIN, Xiao Lei Zhang, Shen Liu, Yan Hao, Zhi Jian Wei, and Shi Qing Feng, “iTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 8, pp. 832–841, 2018, [Online]. Available: https://sid.ir/paper/725725/en

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