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Information Journal Paper

Title

The osteogenesis of bacterial cellulose scaffold loaded with fisetin

Pages

  965-971

Abstract

 Objective(s): Bacterial cellulose (BC) has applications in medical science, it is easily synthesized, economic and purer compared to plant cellulose. The present study aimed to evaluate BC, a biocompatible natural polymer, as a scaffold for the bone marrow Mesenchymal stem cells (BMSCs) loaded with Fisetin, a phytoestrogen. Materials and Methods: BC hydrogel scaffold was prepared from Gluconaceter xylinus and characterized through scanning electron microscopy (SEM). Biocompatibility of BC was measured by MTT assay, BMSCs were obtained from femur of rat and the osteogenic potential of the BC scaffold cultured with BMSCs and loaded with Fisetin, was investigated by measuring the alkaline phosphatase (ALP) activity, alizarin red staining (ARS) and real-time PCR in terms of osteoblast-specific marker, osteocalcin (OCN) and osteopontin (OPN). Results: Biocompatibility results did not show any toxic effects of BC scaffold on BMSCs, while it increased cell viability. The data showed that BC loaded Fisetin differentiated BMSCs into osteoblasts as demonstrated by ALP activity assays and ARS in vitro. Moreover, results from gene expression assay showed the expression of OCN and OPN genes was increased in cells that were seeded on the BC scaffold loaded with Fisetin. Conclusion: According to the results of the present study, BC loaded with Fisetin is an effective strategy to promote osteogenic differentiation and a proper localized delivery system, which could be a potential candidate in bone tissue engineering.

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    Cite

    APA: Copy

    Vadaye Kheiry, Elahe, PARIVAR, KAZEM, BAHARARA, JAVAD, FAZLY BAZZAZ, BIBI SEDIGHEH, & IRANBAKHSH, ALIREZA. (2018). The osteogenesis of bacterial cellulose scaffold loaded with fisetin. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(9), 965-971. SID. https://sid.ir/paper/725800/en

    Vancouver: Copy

    Vadaye Kheiry Elahe, PARIVAR KAZEM, BAHARARA JAVAD, FAZLY BAZZAZ BIBI SEDIGHEH, IRANBAKHSH ALIREZA. The osteogenesis of bacterial cellulose scaffold loaded with fisetin. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(9):965-971. Available from: https://sid.ir/paper/725800/en

    IEEE: Copy

    Elahe Vadaye Kheiry, KAZEM PARIVAR, JAVAD BAHARARA, BIBI SEDIGHEH FAZLY BAZZAZ, and ALIREZA IRANBAKHSH, “The osteogenesis of bacterial cellulose scaffold loaded with fisetin,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 9, pp. 965–971, 2018, [Online]. Available: https://sid.ir/paper/725800/en

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