مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Phospholipase-Cγ 1 Signaling Protein Down-Regulation by Oligoclonal-VHHs based Immuno-Liposome: A Potent Metastasis Deterrent in HER2 Positive Breast Cancer Cells

Pages

  30-39

Abstract

 Objective: The purpose of this study was to develop multivalent antibody constructs via grafting anti-HER2 antibodies, including Herceptin and Oligoclonal-variable domain of heavy chain antibodies (VHHs), onto Liposome membranes to enhance antibody activity and compare their effect on phospholipase C (PLC) signaling pathway with control. Materials and Methods: In this experimental study, SKBR3 and BT-474 cell lines as HER2 positive and MCF10A cell line as normal cell were screened with anti-HER2 antibodies, including constructs of multivalent liposomal antibody developed with Herceptin and anti-HER2 Oligoclonal-VHHs. To confirm the accuracy of the study, immunofluorescent assay, migration assay and immuno-Liposome binding ability to HER2 were evaluated. Finally, the antibodies effect on PLCγ 1 protein level was measured by an immunoassay method (ELISA). Results: In the present study, by using multivalent form of antibodies, we were able to significantly inhibit the PLCγ 1 protein level. Interestingly, the results of migration assay, used for study the motility of different types of cell, shows correspondingly decreased number of immigrated cells in SKBR3 and BT-474 cell lines. Since MCF10A cells show no overexpression of HER2, as expected, the result did not show any change in PLCγ 1 level. Moreover, immunofluorescent assay has confirmed high expression of HER2 in SKBR3 and BT-474 cell lines and low HER2 expression on MCF10A cell line. High binding of immuno-Liposome to SKBR3 and BT-474 cells and low binding to MCF10A confirmed that in this study anti-HER2 antibodies have conserved binding ability to HER2 even after conjugation with Liposome. Conclusion: PLCγ 1 protein levels did indeed decrease after treatment with immuno-Liposome form of compounds in both two tested cell lines, verifying the inhibition ability of them. Moreover, an elevated antibody activity is associated with Liposomes conjugation suggesting that immuno-Liposome may be a potential target for enhancing the antibody activity.

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    Cite

    APA: Copy

    ASADPOUR, OMMOLBANIN, & RAHBARIZADEH, FATEMEH. (2020). Phospholipase-Cγ 1 Signaling Protein Down-Regulation by Oligoclonal-VHHs based Immuno-Liposome: A Potent Metastasis Deterrent in HER2 Positive Breast Cancer Cells. CELL JOURNAL (YAKHTEH), 22(1), 30-39. SID. https://sid.ir/paper/726357/en

    Vancouver: Copy

    ASADPOUR OMMOLBANIN, RAHBARIZADEH FATEMEH. Phospholipase-Cγ 1 Signaling Protein Down-Regulation by Oligoclonal-VHHs based Immuno-Liposome: A Potent Metastasis Deterrent in HER2 Positive Breast Cancer Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(1):30-39. Available from: https://sid.ir/paper/726357/en

    IEEE: Copy

    OMMOLBANIN ASADPOUR, and FATEMEH RAHBARIZADEH, “Phospholipase-Cγ 1 Signaling Protein Down-Regulation by Oligoclonal-VHHs based Immuno-Liposome: A Potent Metastasis Deterrent in HER2 Positive Breast Cancer Cells,” CELL JOURNAL (YAKHTEH), vol. 22, no. 1, pp. 30–39, 2020, [Online]. Available: https://sid.ir/paper/726357/en

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