Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor

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ZHOU DAN; Cheng Hongjing; Liu Jinyu; ZHANG LEI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:6 Page(s): 662-675

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Title

Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor

Author(s)

ZHOU DAN | Cheng Hongjing | Liu Jinyu | ZHANG LEI | Issue Writer Certificate

Keywords

Hair follicle

HGF

Lentivirus

Liver diseases

Stem cells

Abstract

Objective(s): Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. Materials and Methods: We utilized the gene recombination technology to obtain the human hair mesenchymal Stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry. Results: We show here establishment of the hHGF-overexpressing Lentivirus vector, and successfully transfection to human Hair follicle mesenchymal Stem cells. The verified experiments could demonstrate the human Hair follicle mesenchymal Stem cells which have been transfected still have the properties of Stem cells. Conclusion: We successfully constructed human Hair follicle mesenchymal Stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells.

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APA: Copy

ZHOU, DAN, Cheng, Hongjing, Liu, Jinyu, & ZHANG, LEI. (2017). Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(6), 662-675. SID. https://sid.ir/paper/726649/en

Vancouver: Copy

ZHOU DAN, Cheng Hongjing, Liu Jinyu, ZHANG LEI. Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(6):662-675. Available from: https://sid.ir/paper/726649/en

IEEE: Copy

DAN ZHOU, Hongjing Cheng, Jinyu Liu, and LEI ZHANG, “Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 6, pp. 662–675, 2017, [Online]. Available: https://sid.ir/paper/726649/en

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