The expression level of hsa‑ miR‑ 146a‑ 5p in plasma‑ derived exosomes of patients with diffuse large B‑ cell lymphoma

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zare Nasrin; ESKANDARI NAHID; MEHRZAD VALIOLLAH; HAGHJOOY JAVANMARD SHAGHAYEGH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS) Year:2019 | Volume:24 | Issue:2 Page(s): 0-0

Download Full-Text

View:

215

Download:

202

Cites:

Information Journal Paper

Title

The expression level of hsa‑ miR‑ 146a‑ 5p in plasma‑ derived exosomes of patients with diffuse large B‑ cell lymphoma

Author(s)

Zare Nasrin | ESKANDARI NAHID | MEHRZAD VALIOLLAH | HAGHJOOY JAVANMARD SHAGHAYEGH | Issue Writer Certificate

Keywords

Diffuse large B‑cell lymphoma

exosomes

miR‑146a

Abstract

Background: The standard treatment for patients with diffuse large B‑ cell lymphoma (DLBCL) had been rituximab‑ based immunochemotherapy. However, the biological and clinical heterogeneity within DLBCL seems to affect treatment outcome. Therefore, the evaluation of miRNA levels might be useful in predicting treatment response and relapse risk. miR‑ 146a is a modulator of innate and acquired immunity and may play an important role in predicting treatment response. The aim of the present study was to compare the expression level of miR‑ 146a in plasma‑ derived exosomes of responsive DLBCL patients (response to R‑ CHOP (Rituximab, and Cyclophosphamide, Hydroxydaunorubicin, Oncovine and Prednisone)), refractory DLBCL patients (resistant to R‑ CHOP), patients receiving R‑ CHOP, and healthy donors. Materials and Methods: After the preparation of plasma and isolation of exosomes, the presence of plasma‑ derived exosome was confirmed by Zetaseizer, electron microscope, and Western blot. The patients’ medical records were collected and analyzed. The expression level of exosomal miR‑ 146a was evaluated in DLBCL patients and healthy donors using real‑ time polymerase chain reaction (PCR). The − Δ Ct values of miR‑ 146a were compared among responsive patients (n = 17), refractory patients (n = 16), patients receiving R‑ CHOP therapy (n = 15), and healthy donors (n = 6). Results: The presence and size of plasma‑ derived exosomes were confirmed. Our findings did not show any significant difference in the expression level of exosomal miR‑ 146a between DLBCL patients and healthy donors (P = 0. 48). As well, the clinical and histopathological parameters were not correlated with the expression level of exosomal miR‑ 146a or plasma miR‑ 146a. The expression level of plasma miR‑ 146 was lower than the expression level of exosomal miR‑ 146 (P = 0. 01). Conclusion: Exosomal miR‑ 146a might be useful as a promising “ liquid biopsy” biomarker in predicting treatment response and relapse risk; however, we could not find significant differences due to small sample size.

Cites

No record.

References

Prognostic role of circulating exosomal miRNAs in multiple myeloma

Cite

APA: Copy

Zare, Nasrin, ESKANDARI, NAHID, MEHRZAD, VALIOLLAH, & HAGHJOOY JAVANMARD, SHAGHAYEGH. (2019). The expression level of hsa‑ miR‑ 146a‑ 5p in plasma‑ derived exosomes of patients with diffuse large B‑ cell lymphoma. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 24(2), 0-0. SID. https://sid.ir/paper/726758/en

Vancouver: Copy

Zare Nasrin, ESKANDARI NAHID, MEHRZAD VALIOLLAH, HAGHJOOY JAVANMARD SHAGHAYEGH. The expression level of hsa‑ miR‑ 146a‑ 5p in plasma‑ derived exosomes of patients with diffuse large B‑ cell lymphoma. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)[Internet]. 2019;24(2):0-0. Available from: https://sid.ir/paper/726758/en

IEEE: Copy

Nasrin Zare, NAHID ESKANDARI, VALIOLLAH MEHRZAD, and SHAGHAYEGH HAGHJOOY JAVANMARD, “The expression level of hsa‑ miR‑ 146a‑ 5p in plasma‑ derived exosomes of patients with diffuse large B‑ cell lymphoma,” JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), vol. 24, no. 2, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/726758/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops