DOWN-REGULATION OF MICRORNA-23B AGGRAVATES LPS-INDUCED INFLAMMATORY INJURY IN CHONDROGENIC ATDC5 CELLS BY TARGETING PDCD4

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YANG ZHONGMENG; TANG YUXING; ZHAO QING; LU HUADING; XU GUOYONG

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Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:5 Page(s): 529-535

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Title

DOWN-REGULATION OF MICRORNA-23B AGGRAVATES LPS-INDUCED INFLAMMATORY INJURY IN CHONDROGENIC ATDC5 CELLS BY TARGETING PDCD4

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Keywords

INFLAMMATORY INJURY

MICRORNA-23B

NF-κB/NOTCH

OSTEOARTHRITIS

PDCD4

Abstract

Objective (s): OSTEOARTHRITIS (OA), characterized by degradation of articular cartilage, is a leading cause of disability. As the only cell type present in cartilage, chondrocytes play curial roles in the progression of OA. In our study, we aimed to explore the roles of miR-23b in the lipopolysaccharide (LPS) -induced INFLAMMATORY INJURY.Materials and Methods: LPS-induced cell injury of ATDC5 cells was evaluated by the loss of cell viability, enhancement of cell apoptosis, alteration of apoptosis-associated proteins, and release of inflammatory cytokines. Then, miR-23b level after LPS treatment was assessed by qRT-PCR. Next, the effects of aberrantly expressed miR-23b on the LPS-induced INFLAMMATORY INJURY were explored. The possible target genes of miR-23b were virtually screened by informatics and verified by luciferase assay. Subsequently, whether miR- 23b functioned through regulating the target gene was validated. The involved signaling pathways were investigated finally.Results: Cell viability was decreased but cell apoptosis, as well as release of inflammatory cytokines, was enhanced by LPS treatment. MiR-23b was down-regulated by LPS and its overexpression alleviated LPS-induced INFLAMMATORY INJURY. PDCD4, negatively regulated by miR-23b expression, was verified as a target gene of miR-23b. Following experiments showed miR-23b alleviated LPS-induced cell injury through down-regulating PDCD4 expression. Phosphorylated levels of key kinases in the NF-kB pathway, as well as expressions of key kinases in the Notch pathways, were increased by PDCD4 overexpression.Conclusion: MiR-23b was down-regulated after LPS treatment, and its overexpression ameliorated LPS-induced INFLAMMATORY INJURY in ATDC5 cells by targeting PDCD4, which could activate the NF-kB/ Notch pathways.

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APA: Copy

YANG, ZHONGMENG, TANG, YUXING, ZHAO, QING, LU, HUADING, & XU, GUOYONG. (2018). DOWN-REGULATION OF MICRORNA-23B AGGRAVATES LPS-INDUCED INFLAMMATORY INJURY IN CHONDROGENIC ATDC5 CELLS BY TARGETING PDCD4. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(5), 529-535. SID. https://sid.ir/paper/727658/en

Vancouver: Copy

YANG ZHONGMENG, TANG YUXING, ZHAO QING, LU HUADING, XU GUOYONG. DOWN-REGULATION OF MICRORNA-23B AGGRAVATES LPS-INDUCED INFLAMMATORY INJURY IN CHONDROGENIC ATDC5 CELLS BY TARGETING PDCD4. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(5):529-535. Available from: https://sid.ir/paper/727658/en

IEEE: Copy

ZHONGMENG YANG, YUXING TANG, QING ZHAO, HUADING LU, and GUOYONG XU, “DOWN-REGULATION OF MICRORNA-23B AGGRAVATES LPS-INDUCED INFLAMMATORY INJURY IN CHONDROGENIC ATDC5 CELLS BY TARGETING PDCD4,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 5, pp. 529–535, 2018, [Online]. Available: https://sid.ir/paper/727658/en

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