Biosynthesis of Silver Nanoparticles Using Bacteria Isolates from Wastewater

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KAZEMI SHIMA; DOUDI MONIR; Amiri Gholm Reza

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Journal Paper

Paper Information

Journal: MEDICAL LABORATORY JOURNAL Year:2017 | Volume:11 | Issue:6 Page(s): 42-47

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Title

Biosynthesis of Silver Nanoparticles Using Bacteria Isolates from Wastewater

Author(s)

KAZEMI SHIMA | DOUDI MONIR | Amiri Gholm Reza | Issue Writer Certificate

Keywords

Bacteria

Biosynthesis

Minimum Inhibitory Concentration

Abstract

Background and Objectives: Development of ecofriendly processes for the synthesis of metal nanoparticles is of great importance in the field of nanotechnology. Microorganisms such as Bacteria could be suitable candidates for bioproduction of nanoparticles due to their simplicity and high compatibility with the environment. The aim of this study was to use Bacteria isolates from the effluent of wastewater treatment plants to produce silver nanoparticles. Methods: For identifying silver-resistant microorganisms, we used the agar diffusion method using PHG II medium containing 0. 5 mM silver to determine Minimum Inhibitory Concentration. Bacterial identification was done with biochemical testing and polymerase chain reaction (colony PCR). Finally, silver nanoparticles were produced in the desired Bacteria, and the properties of these nanoparticles were studied. Results: We found five silver-resistant Bacteria among which Stenotrophomonas maltophilia strain MS8 showed the highest resistance (MIC= 6 mM). The bacterium was able to synthesize silver nanoparticles in spherical shapes. The results obtained from visual observations using UV-VIS, TEM and XRD showed that the bacterium was able to reduce silver ions into silver nanoparticles with maximum size of 20 nm. Conclusion: Based on our findings, this bacterium could be useful for Biosynthesis of silver nanoparticles.

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APA: Copy

KAZEMI, SHIMA, DOUDI, MONIR, & Amiri, Gholm Reza. (2017). Biosynthesis of Silver Nanoparticles Using Bacteria Isolates from Wastewater. MEDICAL LABORATORY JOURNAL, 11(6), 42-47. SID. https://sid.ir/paper/729376/en

Vancouver: Copy

KAZEMI SHIMA, DOUDI MONIR, Amiri Gholm Reza. Biosynthesis of Silver Nanoparticles Using Bacteria Isolates from Wastewater. MEDICAL LABORATORY JOURNAL[Internet]. 2017;11(6):42-47. Available from: https://sid.ir/paper/729376/en

IEEE: Copy

SHIMA KAZEMI, MONIR DOUDI, and Gholm Reza Amiri, “Biosynthesis of Silver Nanoparticles Using Bacteria Isolates from Wastewater,” MEDICAL LABORATORY JOURNAL, vol. 11, no. 6, pp. 42–47, 2017, [Online]. Available: https://sid.ir/paper/729376/en

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