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Information Journal Paper

Title

Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells

Pages

  236-244

Abstract

 Objective: Mesenchymal stem cells (MSCs) have prominent immunomodulatory roles in the tumor microenvironment. The current study intended to elucidate Treg subsets and their cytokines after exposing naï ve T lymphocytes to adiposederived MSCs (ASCs). Materials and Methods: In this experimental study, to obtain ASCs, breast adipose tissues of a Breast Cancer patient and a normal individual were used. Magnetic cell sorting (MACS) was employed for purifying naï ve CD4+ T cells from peripheral blood of five healthy donors. Naï ve CD4+ T cells were then co-cultured with ASCs for five days. The phenotype of Regulatory T Cells (Tregs) and production of interleukine-10 (IL-10), transforming growth factor beta (TGF-β ) and IL-17 were assessed using flow cytometry and ELISPOT assays, respectively. Results: CD4+CD25-FOXP3+CD45RA+ Tregs were expanded in the presence of cancer ASCs but CD4+CD25+Foxp3+CD45RA+ Regulatory T Cells were up-regulated in the presence of both cancer-and normal-ASCs. This up-regulation was statistically significant in Breast Cancer-ASCs compared to the cells cultured without ASCs (P=0. 002). CD4+CD25+ FOXP3+Helios+, CD4+CD25-FOXP3+Helios+ and CD25+ FOXP3+CD73+CD39+ Tregs were expanded after co-culturing of T cells with both cancer-ASCs and normal-ASCs, while they were statistically significant only in the presence of cancer-ASCs (P<0. 05). Production of IL-10, IL-17 and TGF-β by T cells was increased in the presence of either normal-or cancer-ASCs; however, significant effect was only observed in the IL-10 and TGF-β of cancer-ASCs (P<0. 05). Conclusion: The results further confirm the immunosuppressive impacts of ASCs on T lymphocytes and direct them to specific regulatory phenotypes which may support immune evasion and tumor growth.

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    Cite

    APA: Copy

    Fakhimi, Maryam, TALEI, ABDOLRASOUL, GHADERI, ABBAS, HABIBAGAHI, MOJTABA, & RAZMKHAH, MAHBOOBEH. (2020). Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells. CELL JOURNAL (YAKHTEH), 22(2), 236-244. SID. https://sid.ir/paper/730590/en

    Vancouver: Copy

    Fakhimi Maryam, TALEI ABDOLRASOUL, GHADERI ABBAS, HABIBAGAHI MOJTABA, RAZMKHAH MAHBOOBEH. Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(2):236-244. Available from: https://sid.ir/paper/730590/en

    IEEE: Copy

    Maryam Fakhimi, ABDOLRASOUL TALEI, ABBAS GHADERI, MOJTABA HABIBAGAHI, and MAHBOOBEH RAZMKHAH, “Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells,” CELL JOURNAL (YAKHTEH), vol. 22, no. 2, pp. 236–244, 2020, [Online]. Available: https://sid.ir/paper/730590/en

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