MOLECULAR IDENTIFICATION OF VIRULENCE GENES (AGFA AND MGTC) IN SALMONELLA TYPHIMURIUM STRAINS ISOLATED FROM CHILDREN WITH GASTROENTERITIS USING MULTIPLEX PCR METHOD AND DETERMINATION OF THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN

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AMIRI S.; MORADLI GH.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2016 | Volume:18 | Issue:10 Page(s): 40-45

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Title

MOLECULAR IDENTIFICATION OF VIRULENCE GENES (AGFA AND MGTC) IN SALMONELLA TYPHIMURIUM STRAINS ISOLATED FROM CHILDREN WITH GASTROENTERITIS USING MULTIPLEX PCR METHOD AND DETERMINATION OF THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN

Author(s)

AMIRI S. | MORADLI GH. | Issue Writer Certificate

Keywords

SALMONELLA TYPHIMURIUMQ2

MGTC AND AGFA GENESQ1

ANTIBIOTIC SUSCEPTIBILITYQ2

Abstract

BACKGROUND AND OBJECTIVE: Gastroenteritis caused by salmonella is common in humans and is regarded as a global public health issue. The aim of this study is to identify agfA and mgtC genes in SALMONELLA TYPHIMURIUM strains isolated from stool samples using multiplex PCR method and to determine the resistance patterns of these strains.METHODS: This cross-sectional study was conducted on SALMONELLA TYPHIMURIUM isolated from children with gastroenteritis admitted to Children's Hospital Medical Center in Tehran. Frequency of agfA and mgtC genes was evaluated using multiplex PCR method. In addition, ANTIBIOTIC SUSCEPTIBILITY of these isolates was studied using gel diffusion method and according to CLSI guidelines.FINDINGS: Of total 200 stool samples, 60 SALMONELLA TYPHIMURIUM isolates were obtained. Molecular analysis showed that 24 isolates contained both agfA and mgtC genes at the same time, while 52 isolates contained mgtC gene and 40 isolates carried agfA gene. All strains (100%) were susceptible to ciprofloxacin and 85% of strains were resistant to nitrofurantoin.CONCLUSION: Results of the study demonstrated that most frequent virulence gene in these strains was mgtC (86.6%) and the least frequent virulence gene was agfA (66.6%). Moreover, it was concluded that these isolates were 100% susceptible to ciprofloxacin and were the most resistant to nitrofurantoin (85%).

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APA: Copy

AMIRI, S., & MORADLI, GH.. (2016). MOLECULAR IDENTIFICATION OF VIRULENCE GENES (AGFA AND MGTC) IN SALMONELLA TYPHIMURIUM STRAINS ISOLATED FROM CHILDREN WITH GASTROENTERITIS USING MULTIPLEX PCR METHOD AND DETERMINATION OF THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 18(10), 40-45. SID. https://sid.ir/paper/73232/en

Vancouver: Copy

AMIRI S., MORADLI GH.. MOLECULAR IDENTIFICATION OF VIRULENCE GENES (AGFA AND MGTC) IN SALMONELLA TYPHIMURIUM STRAINS ISOLATED FROM CHILDREN WITH GASTROENTERITIS USING MULTIPLEX PCR METHOD AND DETERMINATION OF THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2016;18(10):40-45. Available from: https://sid.ir/paper/73232/en

IEEE: Copy

S. AMIRI, and GH. MORADLI, “MOLECULAR IDENTIFICATION OF VIRULENCE GENES (AGFA AND MGTC) IN SALMONELLA TYPHIMURIUM STRAINS ISOLATED FROM CHILDREN WITH GASTROENTERITIS USING MULTIPLEX PCR METHOD AND DETERMINATION OF THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 18, no. 10, pp. 40–45, 2016, [Online]. Available: https://sid.ir/paper/73232/en

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