Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Nsaif Jasim Hayder; Hafidh Rand Riadh; Abdulamir Ahmed Sahib

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:11 Page(s): 1100-1108

Download Full-Text

View:

201

Download:

123

Cites:

Information Journal Paper

Title

Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study

Author(s)

Nsaif Jasim Hayder | Hafidh Rand Riadh | Abdulamir Ahmed Sahib | Issue Writer Certificate

Keywords

Acinetobacter baumannii

Bacteriophages

Drug resistance

Endolysin

Phage therapy

Abstract

Objective(s): Phage therapy is a potential alternative treatment for infections caused by Acinetobacter baumannii, a significant nosocomial pathogen, which has evolved resistance to almost all conventional antimicrobial drugs in poor hygiene and conflicts areas such as Iraq. Materials and Methods: Bacteriophages were isolated to highly resistant isolates of A. baumannii to form therapeutic phage cocktail, and to extract and evaluate native Endolysin activity. Bacterial samples were collected in Al-Imamein Al-kadhimein Medical City Hospital. Phages were isolated from different regions in Baghdad city including (soil, sewage, irrigation channels). Phage Endolysin was extracted from highly lytic phages that produced halo-like appearance around inhibition zone. Results: Up to 23 isolates of extensive-and pan-drug resistant (XDR, PDR) A. baumannii were isolated from patients with various infections, and 136 lytic phages specific to A. baumannii were isolated. Each bacterial isolate was sensitive to at least one lytic phage. Accordingly, a phage cocktail was formulated which remarkably minimized bacterial resistance to lysis by phages when compared to individual lytic phages. And, the phage cocktail succeeded in treating and saving life of all bacteremic mice with A. baumannii versus the non-treated group. In addition, the Endolysin native activity to A. baumannii was evaluated in this study; Endolysin revealed a potent antibacterial activity (> 1 log) reduction of bacterial density in just one hour of Endolysin treatment. Conclusion: The Phage therapy assessed in this study showed an ability to efficiently solve the problems of “ superbug” bacteria by lysing effectively most XDR, PDR bacteria in vitro and in vivo. And, phage cocktail was shown to be superior over single-phage preparations in treating A. baumannii with much less resistance rate to therapeutic phages. Furthermore, intrinsic activity of native Endolysin revealed promising results to tackling superbug pathogens.

Cites

No record.

References

No record.

Cite

APA: Copy

Nsaif Jasim, Hayder, Hafidh, Rand Riadh, & Abdulamir, Ahmed Sahib. (2018). Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(11), 1100-1108. SID. https://sid.ir/paper/734143/en

Vancouver: Copy

Nsaif Jasim Hayder, Hafidh Rand Riadh, Abdulamir Ahmed Sahib. Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(11):1100-1108. Available from: https://sid.ir/paper/734143/en

IEEE: Copy

Hayder Nsaif Jasim, Rand Riadh Hafidh, and Ahmed Sahib Abdulamir, “Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 11, pp. 1100–1108, 2018, [Online]. Available: https://sid.ir/paper/734143/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops