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Information Journal Paper

Title

microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF

Pages

  541-546

Keywords

Nasopharyngeal carcinoma-(NPC) 

Abstract

 Objective(s): microRNA-29 (miR-29) family miRNAs have been mentioned as tumor suppressive genes in several human cancers. The purpose of this study was to investigate the function of miR-29a in nasopharyngeal carcinoma (NPC) cells. Materials and Methods: Human NPC cell line 5-8F was transfected with mimic, inhibitor or scrambled controls specific for miR-29a. Subsequently, cell viability, migration, apoptosis and expression changes of VEGF were assessed by trypan blue staining, MTT assay, transwell assay, flow cytometry, Western blot and RT-qPCR. TargetScan online database was used to predict the targets of miR-29a, and luciferase reporter assay was carried out for testing the targeting relationship between VEGF and miR-29a. Western blot analysis was performed to determine the expression changes of core proteins in PI3K/AKT and JAK/STAT pathways. Results: Overexpression of miR-29a suppressed 5-8F cells viability and relative migration, but increased apoptotic cell rate. Consistently, Bcl-2 was downregulated, Bax was upregulated, and caspase-3 and-9 were cleaved by miR-29a overexpression. VEGF was a target gene of miR-29a. Besides, VEGF silence exerted similar effects like miR-29a, as the viability and migration were repressed and apoptosis was induced. Finally, we found that PI3K/AKT and JAK/STAT pathways were deactivated by miR-29a or VEGF silence. Conclusion: These findings highlighted the tumor suppressive effects of miR-29a on NPC cells, as its overexpression inhibited 5-8F cells viability, migration, and induced apoptosis. miR-29a exerted tumor suppressive functions might be via targeting VEGF and deactivating PI3K/AKT and JAK/STAT pathways.

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    • References

    Cite

    APA: Copy

    Shi, Qingyuan, Dai, Jinhua, & Huang, Lizhen. (2019). microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(5), 541-546. SID. https://sid.ir/paper/734292/en

    Vancouver: Copy

    Shi Qingyuan, Dai Jinhua, Huang Lizhen. microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(5):541-546. Available from: https://sid.ir/paper/734292/en

    IEEE: Copy

    Qingyuan Shi, Jinhua Dai, and Lizhen Huang, “microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 5, pp. 541–546, 2019, [Online]. Available: https://sid.ir/paper/734292/en

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