Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance

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del Carmen Ruelas Cinco Elizabeth; Ruiz Madrigal Bertha; Alfredo Domínguez Rosales Jose; Maldonado Gonzalez Montserrat; De la Cruz Color Lucia; Margarita Ramirez Meza Sandra; Rodrigo Torres Baranda Jose; Martinez Lopez Erika; Helena Hernandez Nazara Zamira

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Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:6 Page(s): 623-630

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Title

Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance

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Keywords

AGER protein human

Insulin resistance

Obesity

Oxidative stress

Receptor for advanced-glycation end products

Abstract

Objective(s): The present study aimed to evaluate the receptor of advanced glycation end-products (RAGE), NF-kB, NRF2 gene expression, and RAGE cell distribution in peripheral blood mononuclear cells (PBMC) in subjects with Obesity and IR compared with healthy subjects. Materials and Methods: The mRNA expression levels of RAGE, NF-kB, NRF2, and GAPDH were determined in PBMC by qPCR in 20 obese (OB), 17 obese with Insulin resistance (OB-IR), and 20 healthy subjects (HS), matched by age and sex. RAGE protein expression and its localization were determined by Western Blot and immunocytochemistry (ICC) analysis, total soluble RAGE (sRAGE) and MCP-1 plasma levels by ELISA. Results: : RAGE, NF-kB, and NRF2 genes mRNA expression in PBMCs did not show variation between groups. RAGE protein was lower in OB and OB-IR groups; RAGE was located predominantly on the cellsurface in the OB-IR group compared to the HS group (22% vs 9. 5%, P<0. 001). OB-IR group showed lower sRAGE plasma levels, and correlated negatively with HOMA-IR, ALT parameters (r=-0. 374, r=-0. 429, respectively), and positively with NFE2L2 mRNA (r= 0. 540) P<0. 05. Conclusion: In this study, OB-IR subjects did not reflect significant differences in gene expression; however, correlations detected between sRAGE, biochemical parameters, and NRF2, besides the predominant RAGE distribution on the cell membrane in PBMC could be evidence of the early phase of the inflammatory cascade and the subsequent damage in specific tissues in subjects with OB-IR.

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APA: Copy

Ruiz Madrigal, Bertha, Alfredo Dom?nguez Rosales, Jose, Maldonado Gonzalez, Montserrat, De la Cruz Color, Lucia, Margarita Ramirez Meza, Sandra, Rodrigo Torres Baranda, Jose, Martinez Lopez, Erika, & Helena Hernandez Nazara, Zamira. (2019). Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(6), 623-630. SID. https://sid.ir/paper/734297/en

Vancouver: Copy

Ruiz Madrigal Bertha, Alfredo Dom?nguez Rosales Jose, Maldonado Gonzalez Montserrat, De la Cruz Color Lucia, Margarita Ramirez Meza Sandra, Rodrigo Torres Baranda Jose, Martinez Lopez Erika, Helena Hernandez Nazara Zamira. Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(6):623-630. Available from: https://sid.ir/paper/734297/en

IEEE: Copy

Bertha Ruiz Madrigal, Jose Alfredo Dom?nguez Rosales, Montserrat Maldonado Gonzalez, Lucia De la Cruz Color, Sandra Margarita Ramirez Meza, Jose Rodrigo Torres Baranda, Erika Martinez Lopez, and Zamira Helena Hernandez Nazara, “Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 6, pp. 623–630, 2019, [Online]. Available: https://sid.ir/paper/734297/en

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