Conditioned medium obtained from human amniotic membranederived mesenchymal stem cell attenuates heart failure injury in rats

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Nasseri Maleki Solmaz; ABOUTALEB NAHID; Nazarinia Donya; Allahverdi Beik Sara; Qolamian Asadollah; NOBAKHT MALIHEH

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Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:11 Page(s): 1253-1258

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Title

Conditioned medium obtained from human amniotic membranederived mesenchymal stem cell attenuates heart failure injury in rats

Author(s)

Keywords

Adipose tissue

derived-mesenchymal stem cells

Apoptosis

Conditioned medium

Fibrosis

Heart failure

Abstract

Objective(s): Heart failure (HF) is one of the leading causes of death worldwide. Due to beneficial effects of stem cells, paracrine secretion of them has recently been used by researchers. The purpose of this study was to investigate the effects of intravenous injection (IV) of Conditioned medium (CM) of human amniotic membrane-derived mesenchymal stem cell (MSC-CM) on HF. Materials and Methods: Male Wistar rats (n=35, 180 g) were randomly divided into five groups: sham, HF, HF+MSC-CM, HF+culture medium and HF+phosphate-buffered saline (PBS). To induce HF, isoproterenol (170 mg/kg/d) was injected subcutaneously for 4 consecutive days. After 28 days, induction of HF was evaluated by echocardiography. A day after echocardiography, 50 μ g culture medium/5 ml PBS in HF+culture medium group, 50 μ g MSC-CM/5 ml PBS in HF+MSC-CM group and 5 ml PBS in HF+PBS group were injected two times for 4 successive days. The echocardiography was performed 4 weeks after the last injection of isoproterenol. To evaluate the Fibrosis, morphology, and cardiac function, Trichrome Masson’ s staining, Hematoxylin and Eosin staining and echocardiography were performed, respectively. Results: CM significantly increased fractional shortening and ejection fraction, and also significantly decreased apoptotic nuclear condensation. Moreover, significant decreased level of Fibrosis and increased level of angiogenesis was observed in the treatment group (P<0. 05). Conclusion: Our results indicated that IV injection of CM has therapeutic effects on HF by reducing Fibrosis and preventing the progression of failure due to its paracrine effects.

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