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Information Journal Paper

Title

Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line

Pages

  376-382

Abstract

 Objective(s): microRNAs are small non-coding molecules that regulate gene expression in various biological processes. T-cell acute lymphoblastic leukemia (T-ALL) is a malignancy accompanied with genetic aberrations and accounts for 20% of children’ s and adult’ s ALL. Notch signaling pathway dysregulation occurs in 60% of T-ALL cases. In the present study, we aimed to determine the relationship between miRNAs and genes involved in Notch signaling pathway. Materials and Methods: Considering the role of the pathway and its down-stream genes in proliferation, differentiation, cell cycle, and apoptosis, NOTCH1, c-Myc, and CCND1 genes were selected as target genes. Using Bioinformatics studies, miR-34a, miR-449a, miR-1827, and miR-106b were selected as miRNAs targeting the above-mentioned genes. We evaluated these genes and miRNAs in T-ALL clinical samples as well as Jurkat cell line, in which NOTCH1 is overexpressed. Results: Quantitative Real-Time PCR indicated that NOTCH1, c-Myc, and CCND1 were overexpressed in samples with decreased expression of miR-34a. In addition, we observed that samples with decreased expression of miR-449a showed increased expression of NOTCH1 and CCND1. Furthermore, we analyzed the expression of miR-1827 and miR-106b, which target c-Myc and CCND1, respectively. We found out that the expression of miR-1827, miR-106b, and their respective target genes were inversely correlated in 80% and 75% of the cases (r=0. 8), respectively. Furthermore, in Jurkat cell line, the expression of target genes was increased while the candidate miRNAs except miR-34a were decreased. Conclusion: These miRNAs can be proposed as Biomarkers and new therapeutic targets in T-ALL patients who have NOTCH1 overexpression.

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    APA: Copy

    Naderi, Tohid, MOHAMMADI YEGANEH, SAMIRA, Mohammadi Hezaveh, Neda, Hadavi, Razie, GHAREHBAGHIAN, AHMAD, Vazifeh Shiran, Nader, FALLAH AZAD, VAHID, & PARYAN, MAHDI. (2020). Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(3), 376-382. SID. https://sid.ir/paper/739056/en

    Vancouver: Copy

    Naderi Tohid, MOHAMMADI YEGANEH SAMIRA, Mohammadi Hezaveh Neda, Hadavi Razie, GHAREHBAGHIAN AHMAD, Vazifeh Shiran Nader, FALLAH AZAD VAHID, PARYAN MAHDI. Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(3):376-382. Available from: https://sid.ir/paper/739056/en

    IEEE: Copy

    Tohid Naderi, SAMIRA MOHAMMADI YEGANEH, Neda Mohammadi Hezaveh, Razie Hadavi, AHMAD GHAREHBAGHIAN, Nader Vazifeh Shiran, VAHID FALLAH AZAD, and MAHDI PARYAN, “Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 3, pp. 376–382, 2020, [Online]. Available: https://sid.ir/paper/739056/en

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