Comparison of Common Molecular Typing Methods for Differentiation of Clostridium difficile Strains in the Study of Hospital Acquired Diarrhea

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZIMIRAD MASOUMEH; ALEBOUYEH MASOUD; RASHIDAN MARJAN; ASLANI MOHAMMAD MEHDI; ZALI MOHAMMAD REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Archives of Clinical Infectious Diseases Year:2018 | Volume:13 | Issue:5 Page(s): 0-0

Download Full-Text

View:

183

Download:

123

Cites:

Information Journal Paper

Title

Comparison of Common Molecular Typing Methods for Differentiation of Clostridium difficile Strains in the Study of Hospital Acquired Diarrhea

Author(s)

Keywords

Genotyping

Ribotyping

Random Amplified Polymorphic DNA Technique

Clostridium difficile

Cross-Contamination

Abstract

Background: Cross-Contamination between patients and the medical imaging device is a worldwide concern. In the current study, the ability of different molecular typing methods for differentiation of Clostridium difficile isolates from patientsand medical devices was compared to show their discriminatory power for molecular epidemiological purposes. Methods: A total of 23 C. difficile strains from fresh stool samples of patients subjected to colonoscopy, medical device, and environmental samples of a gastroenterology unit were used for molecular typing. Similarity of the strains was determined by the polymerase chain reaction (PCR) Ribotyping, capillary gel electrophoresis based-Ribotyping, and RAPD-PCR and ERIC-PCR methods. Phylogenetic analysis of the molecular patterns was done by the GelCompar II software and discriminatory power of the methods was measured using Simpson’ s index diversity. Results: RAPD-PCR and PCR-Ribotyping methods showed the highest discrimination power for differentiation of the studied strains, while Genotyping showed the lowest power. Similarity of C. difficile strains between the patients and medical equipment was detected for the strains presenting PCR-ribotypes B and CE-ribotype 150. Conclusions: Involvement of medical device for transmission of toxigenic strains of C. difficile was determined in this study. Although diversity of C. difficile strains was established in the studied hospital, a discrepancy was detected among these techniques for typing purposes. The results suggested the usage of a combination of two or more typing methods for detection of sources of Cross-Contamination in each hospital.

Cites

No record.

References

No record.

Cite

APA: Copy

AZIMIRAD, MASOUMEH, ALEBOUYEH, MASOUD, RASHIDAN, MARJAN, ASLANI, MOHAMMAD MEHDI, & ZALI, MOHAMMAD REZA. (2018). Comparison of Common Molecular Typing Methods for Differentiation of Clostridium difficile Strains in the Study of Hospital Acquired Diarrhea. ARCHIVES OF CLINICAL INFECTIOUS DISEASES, 13(5), 0-0. SID. https://sid.ir/paper/739073/en

Vancouver: Copy

AZIMIRAD MASOUMEH, ALEBOUYEH MASOUD, RASHIDAN MARJAN, ASLANI MOHAMMAD MEHDI, ZALI MOHAMMAD REZA. Comparison of Common Molecular Typing Methods for Differentiation of Clostridium difficile Strains in the Study of Hospital Acquired Diarrhea. ARCHIVES OF CLINICAL INFECTIOUS DISEASES[Internet]. 2018;13(5):0-0. Available from: https://sid.ir/paper/739073/en

IEEE: Copy

MASOUMEH AZIMIRAD, MASOUD ALEBOUYEH, MARJAN RASHIDAN, MOHAMMAD MEHDI ASLANI, and MOHAMMAD REZA ZALI, “Comparison of Common Molecular Typing Methods for Differentiation of Clostridium difficile Strains in the Study of Hospital Acquired Diarrhea,” ARCHIVES OF CLINICAL INFECTIOUS DISEASES, vol. 13, no. 5, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/739073/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops