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Information Journal Paper

Title

Presynaptic Activity of an Isolated Fraction from Rhinella schneideri Poison

Pages

  517-522

Abstract

 Purpose: Rhinella schneideri is a toad found in many regions of the South America. The poison of the glands has cardiotoxic effect in animals and neuromuscular effects in mice and avian preparation. The purpose of this work was to identify the toxin responsible for the neuromuscular effect in avian and mice neuromuscular preparation. Methods: The methanolic extract from R. schneideri poison was fractioned by reversed phase HPLC. The purity and molecular mass were determined by LC/MS mass spectrometry. Chick biventer cervicis and mouse phrenic-nerve diaphragm were used as neuromuscular preparations to identify the toxin. Results: The purification resulted in 32 fractions, which 4 of them were active in neuromuscular preparation. The toxin of fraction 20 were chosen for better reproducibility of the whole extract activity and its molecular mass was 730. 6 Da. The toxin produced facilitation of the muscle contraction followed by a complete neuromuscular blockade in chick biventer cervicis preparation in 90 min without interfering with the exogenous response to ACh and KCl. The quantal content was increased from 128 ± 13 (control) to 216 ± 44 (after 5 min and sustained until 60 min) in the presence of the toxin. Conclusion: In conclusion, our results demonstrated that the neuromuscular action of the poison of Rhinella schneideri is a multitoxin effect. More, the present work first isolated a 730. 6 Da toxin that better represent the whole poison neuromuscular effect, to which is attributed a presynaptic action in avian and mouse neuromuscular preparation.

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    • Cite

    APA: Copy

    Rostelato Ferreira, Sandro, Dal Belo, Chariston Andre, da Silva Junior, Pedro Ismael, Hyslop, Stephen, Rodrigues Simioni, Lea, & Alves Rocha e Silva, Thomaz Augusto. (2018). Presynaptic Activity of an Isolated Fraction from Rhinella schneideri Poison. ADVANCED PHARMACEUTICAL BULLETIN, 8(3), 517-522. SID. https://sid.ir/paper/740058/en

    Vancouver: Copy

    Rostelato Ferreira Sandro, Dal Belo Chariston Andre, da Silva Junior Pedro Ismael, Hyslop Stephen, Rodrigues Simioni Lea, Alves Rocha e Silva Thomaz Augusto. Presynaptic Activity of an Isolated Fraction from Rhinella schneideri Poison. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2018;8(3):517-522. Available from: https://sid.ir/paper/740058/en

    IEEE: Copy

    Sandro Rostelato Ferreira, Chariston Andre Dal Belo, Pedro Ismael da Silva Junior, Stephen Hyslop, Lea Rodrigues Simioni, and Thomaz Augusto Alves Rocha e Silva, “Presynaptic Activity of an Isolated Fraction from Rhinella schneideri Poison,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 8, no. 3, pp. 517–522, 2018, [Online]. Available: https://sid.ir/paper/740058/en

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