Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran

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ESLAMI GILDA; FATTAHI BAFGHI ALI; LOTFI MOHAMMAD HASSAN; MIRZAEI FARZANEH; AHMADI SOMAYEH; TAJFIROUZEH Ali Akbar; JAFARIZADEH Hamid; PORMAZAR Sayyed Alireza; VAKILI MAHMOUD

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Journal: Iranian Journal of Public Health Year:2020 | Volume:49 | Issue:5 Page(s): 975-980

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Title

Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran

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Keywords

Cutaneous leishmaniasis

Iran

Abstract

Background: Cutaneous leishmaniasis (CL) is a major health problem in many parts of Iran. Many methods have been introduced for detection and identification of Cutaneous leishmaniasis. The purpose of this study was isolation and molecular identification of Leishmania spp. agents in patients with CL from endemic region of central Iran. In this study, one of the main loci of central Iran named Yazd will be assessed CL identification using PCR-RFLP. Methods: For this cross-sectional study, sampling was done from 372 suspicious patients with CL who referred to Health Centers of Yazd Province from 2016 to 2017. After collection samples of patients, DNA extraction was done from samples on slides. Genus detection was done using specific primers by PCR. RFLP analysis was done for species identification. Results: Out of 372 samples, 159 samples were positive using PCR based method. Out of 159 samples, 87 (54. 7%) L. major and 72 (45. 3%) L. tropica were identified using RFLP analysis. The number of lesions in each patient was different but 119 (74. 8%) patients showed the number of 1-3 lesions, and more lesions (more than 10 lesions) was showed in 4 (2. 5%) person. Conclusion: The CL found in Yazd province resulted from L. major and L. tropica as the agents of rural and urban types, respectively. The prevalence of L. major and L. tropica was almost the same. This indicated that control programs could be designed for treatment and vector and reservoir control.

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APA: Copy

ESLAMI, GILDA, FATTAHI BAFGHI, ALI, LOTFI, MOHAMMAD HASSAN, MIRZAEI, FARZANEH, AHMADI, SOMAYEH, TAJFIROUZEH, Ali Akbar, JAFARIZADEH, Hamid, PORMAZAR, Sayyed Alireza, & VAKILI, MAHMOUD. (2020). Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran. IRANIAN JOURNAL OF PUBLIC HEALTH, 49(5), 975-980. SID. https://sid.ir/paper/740243/en

Vancouver: Copy

ESLAMI GILDA, FATTAHI BAFGHI ALI, LOTFI MOHAMMAD HASSAN, MIRZAEI FARZANEH, AHMADI SOMAYEH, TAJFIROUZEH Ali Akbar, JAFARIZADEH Hamid, PORMAZAR Sayyed Alireza, VAKILI MAHMOUD. Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2020;49(5):975-980. Available from: https://sid.ir/paper/740243/en

IEEE: Copy

GILDA ESLAMI, ALI FATTAHI BAFGHI, MOHAMMAD HASSAN LOTFI, FARZANEH MIRZAEI, SOMAYEH AHMADI, Ali Akbar TAJFIROUZEH, Hamid JAFARIZADEH, Sayyed Alireza PORMAZAR, and MAHMOUD VAKILI, “Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 49, no. 5, pp. 975–980, 2020, [Online]. Available: https://sid.ir/paper/740243/en

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