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Information Journal Paper

Title

Anti-proliferative and Anti-metastatic Potential of Curcumin Analogue, Pentagamavunon-1 (PGV-1), Toward Highly Metastatic Breast Cancer Cells in Correlation with ROS Generation

Pages

  445-452

Keywords

PGV-1 (curcumin analogue) 
Reactive oxygen species (ROS) 

Abstract

 Purpose: Pentagamavunon-1 (PGV-1) is a curcumin analogue that shows cytotoxic activity in various cancer cells. In this study, we evaluated the effect of PGV-1 on a highly metastatic breast cancer cell line, The 4T1 cells, as an anti-metastatic and Anti-proliferative agent. Methods: Cell viability was evaluated using MTT assay; while cell cycle profile, apoptosis incidence, and ROS intracellular level were determined by flow cytometry. Cell senescence was observed under senescence-associated-β-galactosidase (SA-β-gal) staining assay. The expression of matrixmetalloproteinase-9 (MMP-9) was determined using immunoreaction based-ELISA, while other proteins expression were detected using immunoblotting. Results: Curcumin and PGV-1 showed cytotoxic effects on 4T1 cells with IC50 value of 50 and 4 μ M, respectively. The cytotoxic activity of PGV-1 was correlated to the induction of G2/M cell cycle arrest and cell senescence. Furthermore, PGV-1 increased the accumulation of intracellular ROS level. We also revealed that PGV-1 bound to several ROS-metabolizing enzymes, including glyoxalase I (GLO1), peroxiredoxin 1 (PRDX1), N-ribosyldihydronicotinamide: quinone reductase 2 (NQO2), aldo-keto reductase family 1 member c1 (AKR1C1). As an antimetastatic agent, PGV-1 showed less inhibitory effect on cell migration compared to curcumin. However, PGV-1 significantly decreased MMP-9 protein expression in a dose-dependent manner suggesting it still potent to inhibit metastatic cells. Conclusion: Overall, our findings suggest that PGV-1 is potential to be developed as an antiproliferative and anti-metastatic agent.

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    APA: Copy

    MEIYANTO, EDY, Putri, Herwandhani, Larasati, Yonika Arum, Utomo, Rohmad Yudi, JENIE, RIRIS ISTIGHFARI, Ikawati, Muthi, Lestari, Beni, Yoneda Kato, Noriko, Nakamae, Ikuko, Kawaichi, Masashi, & Kato, Jun Ya. (2019). Anti-proliferative and Anti-metastatic Potential of Curcumin Analogue, Pentagamavunon-1 (PGV-1), Toward Highly Metastatic Breast Cancer Cells in Correlation with ROS Generation. ADVANCED PHARMACEUTICAL BULLETIN, 9(3), 445-452. SID. https://sid.ir/paper/740312/en

    Vancouver: Copy

    MEIYANTO EDY, Putri Herwandhani, Larasati Yonika Arum, Utomo Rohmad Yudi, JENIE RIRIS ISTIGHFARI, Ikawati Muthi, Lestari Beni, Yoneda Kato Noriko, Nakamae Ikuko, Kawaichi Masashi, Kato Jun Ya. Anti-proliferative and Anti-metastatic Potential of Curcumin Analogue, Pentagamavunon-1 (PGV-1), Toward Highly Metastatic Breast Cancer Cells in Correlation with ROS Generation. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2019;9(3):445-452. Available from: https://sid.ir/paper/740312/en

    IEEE: Copy

    EDY MEIYANTO, Herwandhani Putri, Yonika Arum Larasati, Rohmad Yudi Utomo, RIRIS ISTIGHFARI JENIE, Muthi Ikawati, Beni Lestari, Noriko Yoneda Kato, Ikuko Nakamae, Masashi Kawaichi, and Jun Ya Kato, “Anti-proliferative and Anti-metastatic Potential of Curcumin Analogue, Pentagamavunon-1 (PGV-1), Toward Highly Metastatic Breast Cancer Cells in Correlation with ROS Generation,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 9, no. 3, pp. 445–452, 2019, [Online]. Available: https://sid.ir/paper/740312/en

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