Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn

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Min Xiangjing; Guo Yanling; Zhou Yishan; CHEN XIUPING

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Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2021 | Volume:22 | Issue:4 (88) Page(s): 523-531

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Title

Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn

Author(s)

Min Xiangjing | Guo Yanling | Zhou Yishan | CHEN XIUPING | Issue Writer Certificate

Keywords

Dextran Sulfate Sodium

Inflammation

Neferine

Ulcerative Colitis

Abstract

Objective: Ulcerative Colitis (UC) is a long-lasting inflammatory disease of the colon. Epidemiological studies showed that the prevalence and incidence of UC are increasing worldwide in recent years. Neferine is a natural alkaloid isolated from Nelumbo nucifera Gaertn that exerts a variety of biological activities. This study was designed to evaluate the protective effect of Neferine on Dextran Sulfate Sodium (DSS)-induced experimental UC in mice. Materials and Methods: In this experimental study, 4% DSS was used to induce a mice model of UC. Neferine (5 and 10 mg/kg) was administered by intraperitoneal injection (ip). Clinical symptoms and disease activity index (DAI) scores were recorded and calculated. Pathological changes of colon tissues were detected by Hematoxylin and Eosin (H&E) staining. The levels of inflammatory mediators were detected by ELISA kits. Western blotting and immunohistochemical analysis were used for the evaluation of protein expressions. Results: Neferine treatment significantly alleviated DSS-induced UC by inhibiting weight loss, decreasing DAI scores, and alleviating the pathological changes in colon tissues. Furthermore, Neferine significantly decreased serum levels of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-α ), interleukin-1 beta (IL-1β ), and IL-6 and increased serum levels of anti-inflammatory cytokine IL-10. The increased myeloperoxidase (MPO) activity and nitric oxide (NO) in colon tissues were also inhibited. In addition, Neferine significantly down-regulated inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and intercellular cell adhesion molecule-1 (ICAM-1) expression in colon tissues. Conclusion: These results provided evidence that Neferine could protect against DSS-induced UC symptoms in an experimental mice model. This effect might be mediated through inhibition of Inflammation.

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APA: Copy

Min, Xiangjing, Guo, Yanling, Zhou, Yishan, & CHEN, XIUPING. (2021). Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn. CELL JOURNAL (YAKHTEH), 22(4 (88)), 523-531. SID. https://sid.ir/paper/740882/en

Vancouver: Copy

Min Xiangjing, Guo Yanling, Zhou Yishan, CHEN XIUPING. Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn. CELL JOURNAL (YAKHTEH)[Internet]. 2021;22(4 (88)):523-531. Available from: https://sid.ir/paper/740882/en

IEEE: Copy

Xiangjing Min, Yanling Guo, Yishan Zhou, and XIUPING CHEN, “Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn,” CELL JOURNAL (YAKHTEH), vol. 22, no. 4 (88), pp. 523–531, 2021, [Online]. Available: https://sid.ir/paper/740882/en

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