Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

466
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

580
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

The Effect of Arbutin on The Expression of Tumor Suppressor P53, BAX/BCL-2 Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines

Pages

  532-541

Abstract

 Objective: Arbutin (p-hydroxyphenyl-β-D-glucopyranoside) possesses beneficial functions including antioxidant, antiinflammatory, and anti-tumoral activities. Due to the important role of Oxidative Stress and apoptosis in the successful treatment of cancer, understanding mechanisms that lead to apoptosis in cancer cells, is essential. The purpose of the current study was to evaluate the effect of Arbutin on Tert-Butyl Hydroperoxide (t-BHP)-induced Oxidative Stress and the related mechanisms in Fibroblast and Lymph Node Carcinoma of the Prostate (LNCaP) cells. Materials and Methods: In this experimental study, the LNCaP and Fibroblast cell lines were pre-treated with Arbutin (50, 250 and 1000 μ M). After 24 hours, t-BHP (30 and 35 μ M) was added to the cells. Viability was measured (at 24 and 48 hours) using MTT assay. The antioxidant effect of Arbutin was measured by FRAP assay. The mRNA expression of P53 and BAX/BCL-2 ratio were measured using quantitative polymerase chain reaction (PCR). The percentage of apoptotic or necrotic cells was determined using a double staining annexin V fluorescein isothiocyanate (FITC) apoptosis detection kit. Results: Arbutin pre-treatment increased the total antioxidative power and cell viability in the MTT assay and reduced BAX/BCL-2 ratio, P53 mRNA expression and necrosis in Fibroblasts exposed to the oxidative agent (P<0. 001). In addition, our results showed that Arbutin can decrease cell viability, induce apoptosis and increase BAX/BCL-2 ratio in LNCaP cells at some specific concentrations (P<0. 001). Conclusion: Arbutin as a potential functional β-D-glucopyranoside has strong ability to selectively protect Fibroblasts against t-BHP-induced cell damage and induce apoptosis in LNCaP cells.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Ebadollahi, Shima, POURAMIR, MAHDI, ZABIHI, EBRAHIM, GOLPOUR, MONIREH, & AGHAJANPOUR MIR, SEYED MOHSEN. (2021). The Effect of Arbutin on The Expression of Tumor Suppressor P53, BAX/BCL-2 Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines. CELL JOURNAL (YAKHTEH), 22(4 (88)), 532-541. SID. https://sid.ir/paper/740883/en

    Vancouver: Copy

    Ebadollahi Shima, POURAMIR MAHDI, ZABIHI EBRAHIM, GOLPOUR MONIREH, AGHAJANPOUR MIR SEYED MOHSEN. The Effect of Arbutin on The Expression of Tumor Suppressor P53, BAX/BCL-2 Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines. CELL JOURNAL (YAKHTEH)[Internet]. 2021;22(4 (88)):532-541. Available from: https://sid.ir/paper/740883/en

    IEEE: Copy

    Shima Ebadollahi, MAHDI POURAMIR, EBRAHIM ZABIHI, MONIREH GOLPOUR, and SEYED MOHSEN AGHAJANPOUR MIR, “The Effect of Arbutin on The Expression of Tumor Suppressor P53, BAX/BCL-2 Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines,” CELL JOURNAL (YAKHTEH), vol. 22, no. 4 (88), pp. 532–541, 2021, [Online]. Available: https://sid.ir/paper/740883/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top