Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

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RANJBAR REZA; AFSHAR DAVOUD

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Journal Paper

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Journal: Iranian Journal of Public Health Year:2019 | Volume:48 | Issue:10 Page(s): 1879-1884

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Title

Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

Author(s)

RANJBAR REZA | AFSHAR DAVOUD | Issue Writer Certificate

Keywords

Klebsiella pneumoniae

Molecular typing

(GTG) 5-PCR assay

Abstract

Background: Klebsiella pneumonia is one of the common causes of pneumonia and bacteremia in intensive care patients. The present study was aimed to determine the capability of (GTG) 5-PCR assay for Molecular typing of K. pneumonia strains isolated from patients with urinary tract infections. Methods: In this descriptive-sectional study, K. pneumoniae strains were collected from hospitalized patients with urinary tract infection in Baqiyatallah Hospital, Tehran, Iran during 2017-2018. Isolates were identified by conventional microbiological tests. Bacterial DNA was extracted using boiling method and (GTG) 5-PCR assay was used for subtyping of the isolates. For clustering of isolates, dendrogram was generated according to the unweighted pair group method with arithmetic (UPGMA). Results: Overall, 88 K. pneumoniae isolates were isolated and subjected to the Molecular typing study. The (GTG) 5– PCR assay was able to differentiate the K. pneumoniae strains into 9 clusters including G1-G9. Genotype clusters G4 and G9 consist of highest (26) and lowest (1) number isolate, respectively. Conclusion: The K. pneumonia strains isolated under the study belonged to various clones and the (GTG) 5-PCR assay as simple and rapid method can be a powerful tool for Molecular typing of K. pneumoniae strains.

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APA: Copy

RANJBAR, REZA, & AFSHAR, DAVOUD. (2019). Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections. IRANIAN JOURNAL OF PUBLIC HEALTH, 48(10), 1879-1884. SID. https://sid.ir/paper/741541/en

Vancouver: Copy

RANJBAR REZA, AFSHAR DAVOUD. Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2019;48(10):1879-1884. Available from: https://sid.ir/paper/741541/en

IEEE: Copy

REZA RANJBAR, and DAVOUD AFSHAR, “Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 48, no. 10, pp. 1879–1884, 2019, [Online]. Available: https://sid.ir/paper/741541/en

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