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Information Journal Paper

Title

The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature

Pages

  542-547

Abstract

 Objective: MicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. miR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. It has been shown that platelets could be stored in Composol for a longer period of time. The aim of the present study was to assess and compare the expression pattern of miR-16 in platelet concentrates (PCs) in plasma and Composol media. Materials and Methods: In an experimental study, ten PC bags were collected and each bag was divided into two separate bags, one with the 70% Composol and the other with only plasma. Both bags were stored for 7 days at 22˚ C and tested on days 1, 3, 5, and 7 of storage. For each sample, we performed quantitative real-time polymerase chain reaction (qRT-PCR). The water-soluble tetrazolium salt-1 (WST-1) test was used to assess platelet viability in all of the samples. Statistical analysis was done by SPSS and REST software. A P<0. 05 was considered statistically significant. Results: miR-16 was significantly elevated during the storage days, with fold changes of 3. 47 (plasma) and 2. 77 (Composol). The Composol group had significantly decreased miR-16 expression compared with the plasma group. Results of the WST-1 test showed less decrease in optical density (OD) in the Composol group (0. 93 ± 0. 4) during the storage days compared with the plasma group (0. 75 ± 0. 3). Conclusion: Our finding supported results from previous studies that reported an increase in miR-16 expression during Platelet Storage. In addition, miR-16 down-regulation in Composol medium implied that Composol might be a good solution for long-term Platelet Storage because it has the potential to elevate the shelf-life of platelets stored at 22˚ C.

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    Cite

    APA: Copy

    RAJABI, ALI, SHARIFI, ZOHREH, YARI, FATEMEH, Deyhim, Mohammadreza, & Jalili, Mohammadali. (2021). The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature. CELL JOURNAL (YAKHTEH), 22(4 (88)), 542-547. SID. https://sid.ir/paper/742509/en

    Vancouver: Copy

    RAJABI ALI, SHARIFI ZOHREH, YARI FATEMEH, Deyhim Mohammadreza, Jalili Mohammadali. The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature. CELL JOURNAL (YAKHTEH)[Internet]. 2021;22(4 (88)):542-547. Available from: https://sid.ir/paper/742509/en

    IEEE: Copy

    ALI RAJABI, ZOHREH SHARIFI, FATEMEH YARI, Mohammadreza Deyhim, and Mohammadali Jalili, “The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature,” CELL JOURNAL (YAKHTEH), vol. 22, no. 4 (88), pp. 542–547, 2021, [Online]. Available: https://sid.ir/paper/742509/en

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