Immunization against Leishmania major infection in BALB/c mice using a subunit-based DNA vaccine derived from TSA, LmSTI1, KMP11, and LACK predominant antigens

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JAJARMI VAHID; SALEHI SANGANI Ghodratollah; MOHEBALI MEHDI; KHAMESIPOUR ALI; BANDEHPOUR MOJGAN; MAHMOUDI MAHMOUD; Zahedi Zavaram Hadi

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Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:12 Page(s): 1493-1501

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Title

Immunization against Leishmania major infection in BALB/c mice using a subunit-based DNA vaccine derived from TSA, LmSTI1, KMP11, and LACK predominant antigens

Author(s)

Keywords

BALB/c mice

Cytokines

DNA

Epitopes

Leishmania major

Vaccines

Abstract

Objective(s): To design a multivalent DNA vaccine encoding the most immunogenic regions of the Leishmania major antigens including TSA (Thiol-specific antioxidant protein), LmSTI1 (Leishmania major stress-inducible protein1), LACK (Leishmania homologue of receptors for activated C Kinase), and KMP11 (kinetoplastid membrane protein-11) on BALB/c mice. Materials and Methods: The chimeric construct was generated including the most immunogenic Epitopes containing a combination of domains and oligopeptides of the aforementioned genes. The construct was cloned into pcDNA 3. 1 plasmid and named “ pleish-dom. ” Following intramuscular injection of mice, the capability of the vector pleish-dom alone and with pIL-12 (expressing murine IL-12) to raise protective Cytokines and parasite burden was evaluated in the BALB/c mice as a susceptible animal model against L. major. Results: The immunized mice with pleish-dom/pIL-12 showed the highest and the lowest levels of interferon-gamma (IFN-γ ) and interleukin-10 (IL-10), as well as the lowest leishmanin skin test (LST) reactions, were found through 8 weeks post-infection. Conclusion: Although the obtained DNA vaccine from the immunogenic chimeric protein of L. major antigens was able to induce a high level of IFN-γ , it partially protected mice against L. major. However co-administration with pIL-12 led to shift immune response to Th1 phenotype, granuloma formation, and lowered parasite burden, and consequently distinct protection was found.

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APA: Copy

JAJARMI, VAHID, SALEHI SANGANI, Ghodratollah, MOHEBALI, MEHDI, KHAMESIPOUR, ALI, BANDEHPOUR, MOJGAN, MAHMOUDI, MAHMOUD, & Zahedi Zavaram, Hadi. (2019). Immunization against Leishmania major infection in BALB/c mice using a subunit-based DNA vaccine derived from TSA, LmSTI1, KMP11, and LACK predominant antigens. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(12), 1493-1501. SID. https://sid.ir/paper/745288/en

Vancouver: Copy

JAJARMI VAHID, SALEHI SANGANI Ghodratollah, MOHEBALI MEHDI, KHAMESIPOUR ALI, BANDEHPOUR MOJGAN, MAHMOUDI MAHMOUD, Zahedi Zavaram Hadi. Immunization against Leishmania major infection in BALB/c mice using a subunit-based DNA vaccine derived from TSA, LmSTI1, KMP11, and LACK predominant antigens. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(12):1493-1501. Available from: https://sid.ir/paper/745288/en

IEEE: Copy

VAHID JAJARMI, Ghodratollah SALEHI SANGANI, MEHDI MOHEBALI, ALI KHAMESIPOUR, MOJGAN BANDEHPOUR, MAHMOUD MAHMOUDI, and Hadi Zahedi Zavaram, “Immunization against Leishmania major infection in BALB/c mice using a subunit-based DNA vaccine derived from TSA, LmSTI1, KMP11, and LACK predominant antigens,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 12, pp. 1493–1501, 2019, [Online]. Available: https://sid.ir/paper/745288/en

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