The regulatory effect of saffron stigma on the gene expression of the glucose metabolism key enzymes and stress proteins in streptozotocin-induced diabetic rats

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Motamedrad Maryam; Shokouhifar Alireza; HEMMATI MINA; Moossavi Maryam

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2019 | Volume:14 | Issue:3 Page(s): 255-262

Download Full-Text

View:

135

Download:

Cites:

Information Journal Paper

Title

The regulatory effect of saffron stigma on the gene expression of the glucose metabolism key enzymes and stress proteins in streptozotocin-induced diabetic rats

Author(s)

Motamedrad Maryam | Shokouhifar Alireza | HEMMATI MINA | Moossavi Maryam | Issue Writer Certificate

Keywords

Antioxidant

Diabetes

Saffron

Stress protein

Abstract

Oxidative stress plays a crucial role in the pathogenesis of hyperglycemia mediated complications. Since a great number of researches have reported Antioxidant features of Saffron, this study investigated the Antioxidant effect of Saffron stigma extract (SSE) in streptozotocin-induced diabetic rats. Twenty eight diabetic male Wistar rats were divided in four groups containing: two diabetic groups receiving 25 and 100 mg/kg SSE respectively, one diabetic group receiving glibenclamide (0. 6 mg/kg) and one diabetic control group receiving normal saline. Seven healthy adult male Wistar rats were also used as normal control group. After treatment (21 days), fasting blood glucose, insulin, oxidative stress markers, and pancreatic regeneration were assessed. The gene expression level of heat shock factor1, heat shock protein 27, and heat shock protein 70, also glucokinase (GK), and glucose 6-phosphatase (G6Pase) were determined using real-time polymerase chain reaction (RT-PCR). SSE in high dose (100 mg/kg) reduced fasting blood glucose (8. 3 ± 0. 4 mmol/L) compared with diabetic control (24. 6 ± 1. 2 mmol/L) (P < 0. 05). Furthermore, SSE in high dose increased insulin level compared with diabetic control group (12. 7 ± 0. 6 vs 7. 1 ± 0. 3 μ U/mL). RT-PCR analysis revealed decline in mRNA levels of Stress proteins and G6Pase and increase in mRNA level of GK in treatment diabetic groups compared with diabetic control group. Data showed Antioxidant and antidiabetic effects of SSE through altering insulin release and glucose metabolism pathways. Hypoglycemic potential of SSE may be due to change in GK and G6Pase enzymes expression. These findings provide a basis for the therapeutic potential of Saffron in treatment of Diabetes.

Cites

Weak Anti-inflammatory and Anti-cancer Properties of Saffron

References

No record.

Cite

APA: Copy

Motamedrad, Maryam, Shokouhifar, Alireza, HEMMATI, MINA, & Moossavi, Maryam. (2019). The regulatory effect of saffron stigma on the gene expression of the glucose metabolism key enzymes and stress proteins in streptozotocin-induced diabetic rats. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 14(3), 255-262. SID. https://sid.ir/paper/745409/en

Vancouver: Copy

Motamedrad Maryam, Shokouhifar Alireza, HEMMATI MINA, Moossavi Maryam. The regulatory effect of saffron stigma on the gene expression of the glucose metabolism key enzymes and stress proteins in streptozotocin-induced diabetic rats. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2019;14(3):255-262. Available from: https://sid.ir/paper/745409/en

IEEE: Copy

Maryam Motamedrad, Alireza Shokouhifar, MINA HEMMATI, and Maryam Moossavi, “The regulatory effect of saffron stigma on the gene expression of the glucose metabolism key enzymes and stress proteins in streptozotocin-induced diabetic rats,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 14, no. 3, pp. 255–262, 2019, [Online]. Available: https://sid.ir/paper/745409/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops