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Information Journal Paper

Title

Targeted Co-Delivery of Docetaxel and cMET siRNA for Treatment of Mucin1 Overexpressing Breast Cancer Cells

Pages

  383-393

Abstract

 Purpose: Targeted treatment of breast cancer through combination of chemotherapeutic agents and siRNA had been drawing much attention in recent researches. This study was carried out to evaluate mucin1 Aptamer-conjugated Chitosan nanoparticles containing Docetaxel and cMET siRNA on SKBR3 cells. Methods: Nano-drugs were characterized by transmission electron microscope, Zetasizer and loading efficiency calculation. siRNA entrapment onto nanoparticles, stability of siRNA-loaded nanoparticles and conjugation of mucin1 Aptamer to nanoparticles were evaluated via separate electrophoresis. Cellular uptake of the targeted nanoparticles was evaluated through GFP-plasmid expression in mucin1+ SKBR3 vs. mucin1-CHO cells. Protein expression, cell viability and gene expression were assessed by Western Blotting, MTT assay, and Quantitative Real Time-PCR, respectively. Results: Characterization of nano-drugs represented the ideal size (110. 5± 3. 9 nm), zeta potential (11. 6± 0. 8 mV), and loading efficiency of 90. 7% and 88. 3% for siRNA and Docetaxel, respectively. Different gel electrophoresis affirmed the conjugation of Aptamers to nanoparticles and entrapment of siRNA onto nanoparticles. Increased cellular uptake of Aptamer-conjugated nanoparticles was confirmed by GFP expression. cMET gene silencing was confirmed by Western Blotting. The significant (p ≤ 0. 0001) impact of combination targeted therapy vs. control on cell viability was shown. Results of Quantitative Real Time-PCR represented a remarkably decreased (p ≤ 0. 0001) expression of the studied genes involving in tumorigenicity, metastasis, invasion, and angiogenesis (STAT3, IL8, MMP2, MMP9, and VEGF) by targeted combination treatment vs. control. Conclusion: The mucin1 Aptamer-conjugated Chitosan nanoparticles, containing Docetaxel and cMET siRNA, is suggested for treatment of mucin1+ Metastatic breast cancer cells. However, further studies should be conducted on animal models.

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    APA: Copy

    MAJIDI ZOLBANIN, NAIME, JAFARI, REZA, MAJIDI, JAFAR, ATYABI, FATEMEH, YOUSEFI, MEHDI, JADIDI NIARAGH, FARHAD, AGHEBATI MALEKI, LEILI, SOLTANI ZANGBAR, MOHAMMAD SADEGH, & MOHAJJEL NAYEBI, ALIREZA. (2018). Targeted Co-Delivery of Docetaxel and cMET siRNA for Treatment of Mucin1 Overexpressing Breast Cancer Cells. ADVANCED PHARMACEUTICAL BULLETIN, 8(3), 383-393. SID. https://sid.ir/paper/745804/en

    Vancouver: Copy

    MAJIDI ZOLBANIN NAIME, JAFARI REZA, MAJIDI JAFAR, ATYABI FATEMEH, YOUSEFI MEHDI, JADIDI NIARAGH FARHAD, AGHEBATI MALEKI LEILI, SOLTANI ZANGBAR MOHAMMAD SADEGH, MOHAJJEL NAYEBI ALIREZA. Targeted Co-Delivery of Docetaxel and cMET siRNA for Treatment of Mucin1 Overexpressing Breast Cancer Cells. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2018;8(3):383-393. Available from: https://sid.ir/paper/745804/en

    IEEE: Copy

    NAIME MAJIDI ZOLBANIN, REZA JAFARI, JAFAR MAJIDI, FATEMEH ATYABI, MEHDI YOUSEFI, FARHAD JADIDI NIARAGH, LEILI AGHEBATI MALEKI, MOHAMMAD SADEGH SOLTANI ZANGBAR, and ALIREZA MOHAJJEL NAYEBI, “Targeted Co-Delivery of Docetaxel and cMET siRNA for Treatment of Mucin1 Overexpressing Breast Cancer Cells,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 8, no. 3, pp. 383–393, 2018, [Online]. Available: https://sid.ir/paper/745804/en

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