The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs

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HOSSEINI FARASH BIBI RAZIEH; MOHEBALI MEHDI; KAZEMI BAHRAM; HAJJARAN HOMA; FATA ABDOLMAJID; RAOOFIAN REZA; AKHOUNDI BEHNAZ; MOJARRAD MAJID; MASTROENI Pietro; SHARIFI YAZDI MOHAMMAD KAZEM; TANIPOUR MOHAMMAD HOSSEIN

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2020 | Volume:15 | Issue:1 Page(s): 48-56

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Title

The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs

Author(s)

Keywords

rK39 recombinant antigen

Leishmania infantum

Visceral leishmaniasis

Human

Dog

Abstract

Background: Visceral leishmaniasis (VL) is the most severe form of leishmaniasis in Iran with high mortality rates in the case of inaccurate diagnosis and treatment. This study aimed to prepare and evaluate a new rK39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of VL in Humans and Dogs. Methods: rK39-based enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for the detection of anti L. infantum antibodies. We screened 84 Human sera and 87 Dog sera from clinical cases in the endemic area of Meshkin-Shahr, Iran along with 176 sera from healthy controls (collected from 86 Humans and 90 Dogs) during 2013-2016. Results: Using the rK39 ELISA, a sensitivity of 85. 7% (95% CI, 95-99%) and a specificity of 86. 0% (95% CI, 95%-99%) were detected in Human sera at a 1: 800 (cut-off) titer when DAT-confirmed cases were compared with healthy controls; a sensitivity of 96. 6% (95% CI, 95%-99%) and specificity of 94. 4% (95% CI, 95%-99%) were found at a 1: 80 (cut-off) titer compared with DAT. Kappa analysis indicated agreement between the rK39 ELISA and DAT (0. 718) when using Human sera at a 1: 800 (cut-off) titer as well as (0. 910) at a 1: 80 (cut-off) titer when using Dog sera (P<0. 05). Conclusion: New rK39 recombinant antigen from an Iranian strain of Leishmania infantum seems to be used for diagnosis of VL in Humans and Dogs. Further extended field studies are recommended.

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APA: Copy

MOHEBALI, MEHDI, KAZEMI, BAHRAM, HAJJARAN, HOMA, FATA, ABDOLMAJID, RAOOFIAN, REZA, AKHOUNDI, BEHNAZ, MOJARRAD, MAJID, MASTROENI, Pietro, SHARIFI YAZDI, MOHAMMAD KAZEM, & TANIPOUR, MOHAMMAD HOSSEIN. (2020). The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs. IRANIAN JOURNAL OF PARASITOLOGY, 15(1), 48-56. SID. https://sid.ir/paper/745834/en

Vancouver: Copy

MOHEBALI MEHDI, KAZEMI BAHRAM, HAJJARAN HOMA, FATA ABDOLMAJID, RAOOFIAN REZA, AKHOUNDI BEHNAZ, MOJARRAD MAJID, MASTROENI Pietro, SHARIFI YAZDI MOHAMMAD KAZEM, TANIPOUR MOHAMMAD HOSSEIN. The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2020;15(1):48-56. Available from: https://sid.ir/paper/745834/en

IEEE: Copy

MEHDI MOHEBALI, BAHRAM KAZEMI, HOMA HAJJARAN, ABDOLMAJID FATA, REZA RAOOFIAN, BEHNAZ AKHOUNDI, MAJID MOJARRAD, Pietro MASTROENI, MOHAMMAD KAZEM SHARIFI YAZDI, and MOHAMMAD HOSSEIN TANIPOUR, “The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 15, no. 1, pp. 48–56, 2020, [Online]. Available: https://sid.ir/paper/745834/en

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