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Information Journal Paper

Title

Nanocurcumin-Mediated Down-Regulation of Telomerase Via Stimulating TGFβ 1 Signaling Pathway in Hepatocellular Carcinoma Cells

Pages

  171-179

Abstract

 Background: Curcumin, extracted from turmeric, represents enormous potential to serve as an anticancer agent. Telomerase is viewed as a prominent molecular target of curcumin, and transforming growth factor-β 1 (TGFβ 1) has proven to be a major inhibitory signaling pathway for Telomerase activity. In the current study, we aimed to explore suppressive effects of nanocurcumin on Telomerase expression through TGFβ 1 pathway in a Hepatocellular carcinoma cell line (Huh7). Methods: MTT assay was used to determine the effect of nonocurcumin on viability of Huh7 cells. RT-PCR was used to analyze the Gene expression patterns. Results: MTT assay revealed that nanocurcumin acts in a dose-and time-dependent manner to diminish the cell viability. RT-PCR analysis indicated that nanocurcumin results in augmentation of TGFβ 1 72 hours post treatment and leads to the reduction of Telomerase expression 48 and 72 hours post exposure. Also, up-regulation of Smad3 and E2F1 and down-regulation of Smad7 confirmed the effect of nanocurcumin on intermediate components of TGFβ 1 pathway. Furthermore, transfection of the proximal promoter of Telomerase triggered a significant reduction in luciferase activity. Conclusion: The data from the present study lead us to develop a deeper understanding of the mechanisms underlying nanocurcumin-mediated regulation of Telomerase expression, thereby presenting a new perspective to the landscape of using nanocurcumin as a cancer-oriented therapeutic agent.

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    APA: Copy

    SHARIATI, MOLOOD, HAJIGHOLAMI, SAMIRA, VEISI MALEKSHAHI, ZIBA, ENTEZARI, MALIHEH, BODAGHABADI, NARGES, & SADEGHIZADEH, MAJID. (2018). Nanocurcumin-Mediated Down-Regulation of Telomerase Via Stimulating TGFβ 1 Signaling Pathway in Hepatocellular Carcinoma Cells. IRANIAN BIOMEDICAL JOURNAL, 22(3), 171-179. SID. https://sid.ir/paper/747480/en

    Vancouver: Copy

    SHARIATI MOLOOD, HAJIGHOLAMI SAMIRA, VEISI MALEKSHAHI ZIBA, ENTEZARI MALIHEH, BODAGHABADI NARGES, SADEGHIZADEH MAJID. Nanocurcumin-Mediated Down-Regulation of Telomerase Via Stimulating TGFβ 1 Signaling Pathway in Hepatocellular Carcinoma Cells. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(3):171-179. Available from: https://sid.ir/paper/747480/en

    IEEE: Copy

    MOLOOD SHARIATI, SAMIRA HAJIGHOLAMI, ZIBA VEISI MALEKSHAHI, MALIHEH ENTEZARI, NARGES BODAGHABADI, and MAJID SADEGHIZADEH, “Nanocurcumin-Mediated Down-Regulation of Telomerase Via Stimulating TGFβ 1 Signaling Pathway in Hepatocellular Carcinoma Cells,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 3, pp. 171–179, 2018, [Online]. Available: https://sid.ir/paper/747480/en

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