Effects of Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Bakhtari Azizollah; NAZARI SAEEDEH; ALAEE SANAZ; Kargar Abarghouei Elias; MESBAH FAKHRODDIN; MIRZAEI ESMAEIL; MOLAEI MOHAMMAD JAFAR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2020 | Volume:14 | Issue:3 Page(s): 161-170

Download Full-Text

View:

120

Download:

119

Cites:

Information Journal Paper

Title

Effects of Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells

Author(s)

Keywords

Apoptosis

Nanoparticles

Oocytes

Oxidative Stress

Spermatozoa

Abstract

Background: Although application of superparamagnetic iron oxide Nanoparticles (SPIONs) in industry and medicine has increased, their potential toxicity in reproductive cells remains a controversial issue. This study was undertaken to address the response of sperm, oocyte, and resultant blastocyst to dextran-coated SPIONs (D-SPIONs) treatment during murine in vitro fertilization (IVF). Materials and Methods: In this experimental study, murine mature Oocytes were randomly divided into three groups: control, and low-and high-dose groups in which fertilization medium was mixed with 0, 50 and 250 µ g/ml of DSPIONs, respectively. Sperm and/or cumulus oocyte complexes (COCs) were cultured for 4 h in this medium for electron microscopic analysis of sperm and COCs, and assessment of developmental competence and genes expression of Gpx1, Sod1, catalase, Bcl2l1 and Bax in the resultant blastocysts. Results: Ultrastructural study of sperm, oocyte, and granulosa showed destructed mitochondria and membranes in Spermatozoa, vacuolated mitochondria and distorted cristae in Oocytes, and disrupted nuclei and disorganized cell membranes in granulosa in a dose-dependent manner. Data showed that cleavage and blastocyst rates in the 250 µ g/ml of D-SPIONs were significantly lower than in the control group (P<0. 05). Gene expression of GPx1, Sod1, catalase, Bcl2l1 and Bax in resultant blastocysts of the high-dose group and catalase and Bax in resultant blastocysts of the low-dose group, was higher than the controls. Conclusion: There is considerable concern regarding D-SPIONs toxic effects on IVF, and mitochondrial and cell membrane damage in mouse Spermatozoa and Oocytes, which may be related to Oxidative Stress and apoptotic events.

Cites

No record.

References

No record.

Cite

APA: Copy

Bakhtari, Azizollah, NAZARI, SAEEDEH, ALAEE, SANAZ, Kargar Abarghouei, Elias, MESBAH, FAKHRODDIN, MIRZAEI, ESMAEIL, & MOLAEI, MOHAMMAD JAFAR. (2020). Effects of Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 14(3), 161-170. SID. https://sid.ir/paper/750339/en

Vancouver: Copy

Bakhtari Azizollah, NAZARI SAEEDEH, ALAEE SANAZ, Kargar Abarghouei Elias, MESBAH FAKHRODDIN, MIRZAEI ESMAEIL, MOLAEI MOHAMMAD JAFAR. Effects of Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2020;14(3):161-170. Available from: https://sid.ir/paper/750339/en

IEEE: Copy

Azizollah Bakhtari, SAEEDEH NAZARI, SANAZ ALAEE, Elias Kargar Abarghouei, FAKHRODDIN MESBAH, ESMAEIL MIRZAEI, and MOHAMMAD JAFAR MOLAEI, “Effects of Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 14, no. 3, pp. 161–170, 2020, [Online]. Available: https://sid.ir/paper/750339/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops