EVIDENCES FOR A NEW CATION CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE

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FAHANIK BABAEI JAVAD; JAFARI ADELEH; ELIASSI AFSANEH; SAGHIRI REZA

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Journal: Physiology and Pharmacology Year:2013 | Volume:17 | Issue:1 Page(s): 81-93

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Title

EVIDENCES FOR A NEW CATION CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE

Author(s)

FAHANIK BABAEI JAVAD | JAFARI ADELEH | ELIASSI AFSANEH | SAGHIRI REZA | Issue Writer Certificate

Keywords

MITOCHONDRIAQ2

POTASSIUM CHANNELQ2

4-APQ2

MITOCHONDRIAL CATION CHANNELQ2

Abstract

Introduction: Previous studies and our works have indicated several cation channels in the rat brain MITOCHONDRIAl inner membrane. In this work, we report the single-channel characterization of a cation channel from the rat brain MITOCHONDRIAl inner membrane incorporated into a planar lipid bilayer.Methods: After removing and homogenizing the adult rat brain, its supernatant was centrifuged in MSE-digitonin, H2O and Na2CO3, separately. Then, MITOCHONDRIAl inner membrane vesicles were prepared in MSE solution. L-a- Phosphatidylcholine (for preparing membrane lipid bilayer) was extracted from fresh egg yolk. Bilayer lipid membranes were formed in a 150 mm diameter hole. All single channel recordings were filtered at 1 kHz and stored at a sampling rate of 10 kHz for offline analysis by PClamp10. Statistical analysis was performed based on Markov noise free single channel analysis.Results: The channel conductance was 93 pS in 200 mM KCl cis /50 mM KCl trans. The channel open probability appeared voltage-independent at -50 to+40 mV. The main characteristic of channel gating was its marked bursting behavior. Adding 10 mM 4-aminopyridine (4-AP) at positive and negative potentials inhibited the channel activities.Conclusion: These results demonstrate that a new cation channel, present in the brain MITOCHONDRIAl inner membrane, displays different kinetics and biophysical properties than those classically described for brain MITOCHONDRIA.

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APA: Copy

FAHANIK BABAEI, JAVAD, JAFARI, ADELEH, ELIASSI, AFSANEH, & SAGHIRI, REZA. (2013). EVIDENCES FOR A NEW CATION CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE. PHYSIOLOGY AND PHARMACOLOGY, 17(1), 81-93. SID. https://sid.ir/paper/75051/en

Vancouver: Copy

FAHANIK BABAEI JAVAD, JAFARI ADELEH, ELIASSI AFSANEH, SAGHIRI REZA. EVIDENCES FOR A NEW CATION CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2013;17(1):81-93. Available from: https://sid.ir/paper/75051/en

IEEE: Copy

JAVAD FAHANIK BABAEI, ADELEH JAFARI, AFSANEH ELIASSI, and REZA SAGHIRI, “EVIDENCES FOR A NEW CATION CHANNEL IN THE BRAIN MITOCHONDRIAL INNER MEMBRANE,” PHYSIOLOGY AND PHARMACOLOGY, vol. 17, no. 1, pp. 81–93, 2013, [Online]. Available: https://sid.ir/paper/75051/en

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