NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAYARANI NAJARAN ZAHRA; SADEGHNIA HAMID REZA; ASGHARI MOZHGAN; MOUSAVI SEYED HADI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Physiology and Pharmacology Year:2009 | Volume:13 | Issue:3 Page(s): 263-270

Download Full-Text

Persian Verion

View:

1,109

Download:

Cites:

Information Journal Paper

Title

NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH

Author(s)

TAYARANI NAJARAN ZAHRA | SADEGHNIA HAMID REZA | ASGHARI MOZHGAN | MOUSAVI SEYED HADI | Issue Writer Certificate

Keywords

PC12Q3

SERUM/GLUCOSE FREEQ3

TOXICITYQ3

NIGELLA SATIVAQ3

Abstract

Introduction: The Serum/Glucose deprivation -induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. NIGELLA SATIVA L. has been known as a source of antioxidants. To elucidate the neuroprotective actions of N. sativa extract in vitro, we studied the effect of N. sativa extract on cultured PC12 cells under serum/glucose deprivation conditions.Methods: PC12 cells were cultured in DMEM medium containing 10% (v/v) fetal bovine serum, 100 units/ml penicillin, and 100 mg/ml streptomycin. Cells were seeded overnight and then deprived of serum/glucose for 6 and 18 h.Cells were pretreated with different concentrations of N. sativa extract (7.81-250 mg/ml). Cell viability was quantitated by MTT assay. Intracellular ROS production was measured by flow cytometry using 2', 7’-Dichlorofluorescin diacetate (DCF-DA).Results: Depriving the PC-12 cells of serum/glucose caused prominent cell TOXICITY at least after 6 and 18 h.Pretreatment of PC12 cells with N. sativa (7.81-250 mg/ml) could reduce serum/glucose deprivation-induced cytotoicity in PC12 cells after 18 h. The experimental results suggest that N. sativa extract protects the PC12 cells against Serum/Glucose deprivation-induced cytotoxicity.Conclusion: Our findings might raise a possibility of potential therapeutic application of N. sativa extract for preventing and treating cerebral ischemic and neurodegenerative diseases.

Cites

No record.

References

No record.

Cite

APA: Copy

TAYARANI NAJARAN, ZAHRA, SADEGHNIA, HAMID REZA, ASGHARI, MOZHGAN, & MOUSAVI, SEYED HADI. (2009). NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH. PHYSIOLOGY AND PHARMACOLOGY, 13(3), 263-270. SID. https://sid.ir/paper/75061/en

Vancouver: Copy

TAYARANI NAJARAN ZAHRA, SADEGHNIA HAMID REZA, ASGHARI MOZHGAN, MOUSAVI SEYED HADI. NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2009;13(3):263-270. Available from: https://sid.ir/paper/75061/en

IEEE: Copy

ZAHRA TAYARANI NAJARAN, HAMID REZA SADEGHNIA, MOZHGAN ASGHARI, and SEYED HADI MOUSAVI, “NEUROPROTECTIVE EFFECT OF NIGELLA SATIVA HYDRO ALCOHOLIC EXTRACT ON SERUM/GLUCOSE DEPRIVATION INDUCED PC12 CELLS DEATH,” PHYSIOLOGY AND PHARMACOLOGY, vol. 13, no. 3, pp. 263–270, 2009, [Online]. Available: https://sid.ir/paper/75061/en

Related Journal Papers