Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

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Najafi Asl Majid; FARAJI HABIBOLLAH; HOSSEINI TESHNIZI SAEED; SHARIFI SARASIABI KHOJASTEH

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Journal: Jundishapur Journal of Microbiology Year:2020 | Volume:13 | Issue:6 Page(s): 0-0

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Title

Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

Author(s)

Najafi Asl Majid | FARAJI HABIBOLLAH | HOSSEINI TESHNIZI SAEED | SHARIFI SARASIABI KHOJASTEH | Issue Writer Certificate

Keywords

Genotypes

Subtypes

Cryptosporidium

gp60 gene

Diarrhea

Iran

Abstract

Background: Cryptosporidium species are recognized as one of the most important gastrointestinal pathogens of humans and livestock. Objectives: This study aimed to determine the prevalence and sub-Genotypes of Cryptosporidium spp. among diarrheic patients in Bandar Abbas City, Iran. Methods: Diarrheic fecal samples were collected from 170 patients in three hospitals of Bandar Abbas, Iran, from October 2018 to May 2019. Initial parasitological identification of Cryptosporidium spp. was performed by modified Ziehl-Neelsen (ZN) staining. For molecular analysis, the positive specimens and the suspected ones of Cryptosporidium spp. were evaluated by sequence analysis of the 60-kDa glycoprotein gene (gp60). The collected data were analyzed using SPSS software and the relationship between the variables and the presence of Cryptosporidium spp. assessed by the chi-square test. To assess the degree of agreement between PCR and ZN staining, Cohen’ s kappa-index was applied. Results: Of the 170 diarrheic patients, 98 (57. 6%) were male, and 72 (42. 4%) were female. Prevalence of Cryptosporidium spp. by parasitological examination was 1. 8% (3/170). However, using PCR, Cryptosporidium spp. was detected in 12% (6/50) of the positive microscopically samples (3 samples) and 47 suspected specimens. Sequence analysis of the gp60 gene showed that all of the positive isolates were Cryptosporidium parvum in which all Subtypes belonged to allele family IId. Twodistinct nucleotide sequences obtained from this study were deposited in GenBank under the accession numbers MN820453 and MN820454. Conclusions: The predominance of C. parvum (subtype family IId) in this study emphasizes the importance of zoonotic Cryptosporidium transmission in Bandar Abbas, Southern Iran.

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APA: Copy

Najafi Asl, Majid, FARAJI, HABIBOLLAH, HOSSEINI TESHNIZI, SAEED, & SHARIFI SARASIABI, KHOJASTEH. (2020). Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 13(6), 0-0. SID. https://sid.ir/paper/750934/en

Vancouver: Copy

Najafi Asl Majid, FARAJI HABIBOLLAH, HOSSEINI TESHNIZI SAEED, SHARIFI SARASIABI KHOJASTEH. Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2020;13(6):0-0. Available from: https://sid.ir/paper/750934/en

IEEE: Copy

Majid Najafi Asl, HABIBOLLAH FARAJI, SAEED HOSSEINI TESHNIZI, and KHOJASTEH SHARIFI SARASIABI, “Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 13, no. 6, pp. 0–0, 2020, [Online]. Available: https://sid.ir/paper/750934/en

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