Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis

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Abbasi Nejat Ziba; FARAHYAR SHIRIN; FALAHATI MEHRABAN; ASHRAFI KHOZANI MAHTAB; Hosseini Aga Fateme; Faiazy Azam Sadat; Ekhtiari Masoome; Hashemi Hafshenjani Saeideh

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:1 Page(s): 33-41

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Title

Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis

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Keywords

Candida glabrata

Vulvovaginal candidiasis

Candida krusei

Abstract

Background: Vulvovaginal candidiasis (VVC) is an important health problem caused by Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of nonalbicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches. Clinical isolates were analyzed with specific PCR using CGL primers, and the internal transcribed spacer region and the D1-D2 domain of the large-subunit rRNA gene were amplified and sequenced. Susceptibility to amphotericin B, fluconazole, itraconazole, and clotrimazole was determined by the guidelines of the Clinical and Laboratory Standard Institute. Results: In total, 35 non-albicans Candida isolates were identified from VVC patients. The isolates included 27 strains of Candida glabrata (77. 1%), 5 Candida krusei (Pichia kudriavzevii; 14. 3%), 2 Candida kefyr (Kluyveromyces marxianus; 5. 7%), and 1 Candida lusitaniae (Clavispora lusitaniae; 2. 9%). The fungicides itraconazole and amphotericin B were effective against all species. One isolate of C. glabrata showed resistance to fluconazole and clotrimazole, and 26 isolates of C. glabrata indicated dose-dependent susceptibility to fluconazole. C. lusitaniae was susceptible in a dose-dependent manner to fluconazole and resistant to clotrimazole. Conclusions: Non-albicans Candida spp. are common agents of vulvovaginitis, and C. glabrata is the most common species in the tested patients.

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APA: Copy

Abbasi Nejat, Ziba, FARAHYAR, SHIRIN, FALAHATI, MEHRABAN, ASHRAFI KHOZANI, MAHTAB, Hosseini, Aga Fateme, Faiazy, Azam Sadat, Ekhtiari, Masoome, & Hashemi Hafshenjani, Saeideh. (2018). Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis. IRANIAN BIOMEDICAL JOURNAL, 22(1), 33-41. SID. https://sid.ir/paper/751015/en

Vancouver: Copy

Abbasi Nejat Ziba, FARAHYAR SHIRIN, FALAHATI MEHRABAN, ASHRAFI KHOZANI MAHTAB, Hosseini Aga Fateme, Faiazy Azam Sadat, Ekhtiari Masoome, Hashemi Hafshenjani Saeideh. Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(1):33-41. Available from: https://sid.ir/paper/751015/en

IEEE: Copy

Ziba Abbasi Nejat, SHIRIN FARAHYAR, MEHRABAN FALAHATI, MAHTAB ASHRAFI KHOZANI, Aga Fateme Hosseini, Azam Sadat Faiazy, Masoome Ekhtiari, and Saeideh Hashemi Hafshenjani, “Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 1, pp. 33–41, 2018, [Online]. Available: https://sid.ir/paper/751015/en

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