Reduction in the Viability of Human Cervical Cancer HeLa Cell Line via Indirect Co-culture With Amniotic Fluid-Derived Mesenchymal Stem Cells

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Rahmatizadeh Faramarz; Pashaei Asl Fatima; Mohammadi Dehcheshmeh Manijeh; Rahbar Sara; LaleAtaei Maryam; Gholizadeh Ghaleh Aziz Shiva; SOLEIMANI RAD JAFAR

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Journal: International Journal of Women's Health and Reproduction Sciences Year:2020 | Volume:8 | Issue:3 Page(s): 319-327

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Title

Reduction in the Viability of Human Cervical Cancer HeLa Cell Line via Indirect Co-culture With Amniotic Fluid-Derived Mesenchymal Stem Cells

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Keywords

Mesenchymal stem cells

HeLa cells

Amniotic fluid

Co-culture

Cell-based therapy

Abstract

Objectives: This experiment was carried out to evaluate the impacts of unmodified human Amniotic fluid-derived mesenchymal stromal/stem cells (hAF-MSCs) on the viability of HeLa cells, as well as the impact of these cells on the expression of common pro-apoptotic and pro-survival genes in tumor cells by establishing an indirect Co-culture system. Materials and Methods: To this end, an indirect Co-culture system was established, and hAF-MSCs were Co-cultured with HeLa cells at a ratio of 1:2 for five days. The cell viability of Co-cultured tumor cells was determined after the incubation period. Then, several parameters were examined, including the gene expression of tumor protein 53 (TP53), BCL2-associated X protein (BAX), B-cell lymphoma 2 (BCL-2), and cyclin-dependent kinase inhibitor 1A (CDKN1A). Finally, gene regulatory networks were analyzed as well. Results: The results of this study confirmed that the Co-culture of hAF-MSCs with HeLa cells could decrease the viability of tumor cells. The reduction of HeLa cell viability was accompanied by an increase in BAX, TP53, and CDKN1A while a decrease in BCL-2 gene expression. Eventually, the analysis of the regulatory network revealed that the Co-culture of Hela ¬cells with hAF-MSCs activated several transcriptional factors and microRNAs which regulated the expression of these genes. Conclusions: In general, hAF-MSCs exerted the inhibitive effects on the growth of HeLa cells, along with alterations in the expression of common pro-apoptotic and pro-survival genes in a timely and concentration-dependent manner.

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APA: Copy

Pashaei Asl, Fatima, Mohammadi Dehcheshmeh, Manijeh, Rahbar, Sara, LaleAtaei, Maryam, Gholizadeh Ghaleh Aziz, Shiva, & SOLEIMANI RAD, JAFAR. (2020). Reduction in the Viability of Human Cervical Cancer HeLa Cell Line via Indirect Co-culture With Amniotic Fluid-Derived Mesenchymal Stem Cells. INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES, 8(3), 319-327. SID. https://sid.ir/paper/752605/en

Vancouver: Copy

Pashaei Asl Fatima, Mohammadi Dehcheshmeh Manijeh, Rahbar Sara, LaleAtaei Maryam, Gholizadeh Ghaleh Aziz Shiva, SOLEIMANI RAD JAFAR. Reduction in the Viability of Human Cervical Cancer HeLa Cell Line via Indirect Co-culture With Amniotic Fluid-Derived Mesenchymal Stem Cells. INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES[Internet]. 2020;8(3):319-327. Available from: https://sid.ir/paper/752605/en

IEEE: Copy

Fatima Pashaei Asl, Manijeh Mohammadi Dehcheshmeh, Sara Rahbar, Maryam LaleAtaei, Shiva Gholizadeh Ghaleh Aziz, and JAFAR SOLEIMANI RAD, “Reduction in the Viability of Human Cervical Cancer HeLa Cell Line via Indirect Co-culture With Amniotic Fluid-Derived Mesenchymal Stem Cells,” INTERNATIONAL JOURNAL OF WOMEN’S HEALTH AND REPRODUCTION SCIENCES, vol. 8, no. 3, pp. 319–327, 2020, [Online]. Available: https://sid.ir/paper/752605/en

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