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Information Journal Paper

Title

Influence of simvastatin on the biological behavior of nucleus pulposus-derived mesenchymal stem cells

Pages

  1468-1475

Abstract

 Objective(s): This research is to study the influences of different concentrations of Simvastatin on the biological activities of Nucleus pulposus-derived Mesenchymal stem cells (NPMSC). Materials and Methods: NPMSC were cultured with different concentrations of Simvastatin (0, 0. 01, 0. 1, and 1 μ M) and assessed to determine the possible effects of Simvastatin. The cell proliferation was assessed with CCK-8 assay. The flowcytometry and multilineage differentiation were also performed to identify the stem characterization of the cells. The mRNA expressions of aggrecan, collagen type II, glucose transporter 1 (GLUT-1), vascular endothelial growth factor (VEGF) and Hypoxia-inducible factor-1α (HIF-1α ) were determined by qRT-PCR. Results: The results demonstrated that the cells isolated from Nucleus pulposus of healthy Sprague-Dawley (SD) rat met the criteria of MSC. NPMSC could form sunflower-like colonies and strongly expressed stem cell-related genes. In addition, NPMSC showed strong ability of chondrogenic, adipogenic and osteogenic differentiation. Simvastatin at certain range concentrations (0. 01 μ M-0. 1 μ M)) significantly promoted colony-forming rate and cell proliferation, and inhibited cell Apoptosis. Simvastatin could promote expressions of aggrecan, collagen type II, HIF-1α , VEGF and GLUT-1, while 0. 1 μ mol/l concentration reached the maximum effect. Our study further demonstrated that HIF-1α-intermediated signaling pathway might participate in regulating the biological activities of NPMSC. Conclusion: Proper concentration of Simvastatin can promote the biological behavior of NPMSC, and HIF-1α-intermediated signaling pathway might participate in the mechanism.

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    APA: Copy

    Huang, Zenan, Cheng, Xiaofei, Zhao, Jie, Liu, Zhongjun, Wang, Jingcheng, Feng, Xinmin, & ZHANG, LIANG. (2019). Influence of simvastatin on the biological behavior of nucleus pulposus-derived mesenchymal stem cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(12), 1468-1475. SID. https://sid.ir/paper/753143/en

    Vancouver: Copy

    Huang Zenan, Cheng Xiaofei, Zhao Jie, Liu Zhongjun, Wang Jingcheng, Feng Xinmin, ZHANG LIANG. Influence of simvastatin on the biological behavior of nucleus pulposus-derived mesenchymal stem cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(12):1468-1475. Available from: https://sid.ir/paper/753143/en

    IEEE: Copy

    Zenan Huang, Xiaofei Cheng, Jie Zhao, Zhongjun Liu, Jingcheng Wang, Xinmin Feng, and LIANG ZHANG, “Influence of simvastatin on the biological behavior of nucleus pulposus-derived mesenchymal stem cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 12, pp. 1468–1475, 2019, [Online]. Available: https://sid.ir/paper/753143/en

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