Display of human and rabbit monocyte chemoattractant protein-1 on human embryonic kidney 293T cell surface

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BOSHTAM MARYAM; ASGARY SEDDIGHE; RAHIMMANESH ILNAZ; Kouhpayeh Shirin; Naderi Jamal; HEJAZI ZAHRA; Mohammad Dezashibi Hoda; Pieper Ina Laura; KHANAHMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2018 | Volume:13 | Issue:5 Page(s): 430-439

Download Full-Text

View:

123

Download:

Cites:

Information Journal Paper

Title

Display of human and rabbit monocyte chemoattractant protein-1 on human embryonic kidney 293T cell surface

Author(s)

Keywords

Cell surface display

Chemokine CCL2

Flow cytometry

Transfection

Transformation

Abstract

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is a protein that is secreted immediately upon endothelial injury, and thereby it plays a key role in inflammation via recruitment of leucocytes to the site of inflammation at the beginning and throughout the inflammatory processes. Aim of this study was to develop two separate cell lines displaying either human MCP-1 (HMCP-1) or rabbit MCP-1 (RMCP-1) on their surface. A DNA fragment containing HMCP-1-or RMCP-1-encoding sequence was inserted into a pcDNA plasmid. Escherichia coli cells strain TOP 10F' was separately transformed with the pcDNA/RMCP-1 or /HMCP-1 ligation mixture. Following the cloning and construct verification, human embryonic kidney cell line (HEK 293T) was transfected with either of the linearized plasmids. Plasmid integration into the genomic DNA of HEK 293T cells was verified by polymerase chain reaction (PCR). HMCP-1 and RMCP-1 expression was evaluated at RNA and protein levels by real-time PCR and Flow cytometry, respectively. PCR products of the expected sizes were amplified from the chromosomal DNA of transfected HEK 293T cells, i. e. 644 bp for H-MCP1 and 737 bp for RMCP-1 constructs. Real-time PCR revealed that the copy numbers of RMCP1 and HMCP1 mRNA per cell were 294 and 500, respectively. Flow cytometry analysis indicated 85% for RMCP-1 and 87% for HMCP-1 expression levels on the surface of transfected cells, when compared with an isotype control. The experiments thus confirmed that the MCP-1 genes were integrated into the HEK 293T genomic DNA and the encoded proteins were stably expressed on the cell surface.

Cites

No record.

References

No record.

Cite

APA: Copy

BOSHTAM, MARYAM, ASGARY, SEDDIGHE, RAHIMMANESH, ILNAZ, Kouhpayeh, Shirin, Naderi, Jamal, HEJAZI, ZAHRA, Mohammad Dezashibi, Hoda, Pieper, Ina Laura, & KHANAHMAD, HOSSEIN. (2018). Display of human and rabbit monocyte chemoattractant protein-1 on human embryonic kidney 293T cell surface. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 13(5), 430-439. SID. https://sid.ir/paper/753748/en

Vancouver: Copy

BOSHTAM MARYAM, ASGARY SEDDIGHE, RAHIMMANESH ILNAZ, Kouhpayeh Shirin, Naderi Jamal, HEJAZI ZAHRA, Mohammad Dezashibi Hoda, Pieper Ina Laura, KHANAHMAD HOSSEIN. Display of human and rabbit monocyte chemoattractant protein-1 on human embryonic kidney 293T cell surface. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2018;13(5):430-439. Available from: https://sid.ir/paper/753748/en

IEEE: Copy

MARYAM BOSHTAM, SEDDIGHE ASGARY, ILNAZ RAHIMMANESH, Shirin Kouhpayeh, Jamal Naderi, ZAHRA HEJAZI, Hoda Mohammad Dezashibi, Ina Laura Pieper, and HOSSEIN KHANAHMAD, “Display of human and rabbit monocyte chemoattractant protein-1 on human embryonic kidney 293T cell surface,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 13, no. 5, pp. 430–439, 2018, [Online]. Available: https://sid.ir/paper/753748/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops