Detection of Genes Involved in Bioﬁ lm Formation in MDR and XDR Acinetobacter baumannii Isolated from Human Clinical Specimens in Isfahan, Iran

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Mahmoudi Monfared Ahad; REZAEI ALIAKBAR; POURSINA FARKHONDEH; FAGHRI JAMSHID

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Journal: Archives of Clinical Infectious Diseases Year:2019 | Volume:14 | Issue:2 Page(s): 0-0

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Title

Detection of Genes Involved in Bioﬁ lm Formation in MDR and XDR Acinetobacter baumannii Isolated from Human Clinical Specimens in Isfahan, Iran

Author(s)

Mahmoudi Monfared Ahad | REZAEI ALIAKBAR | POURSINA FARKHONDEH | FAGHRI JAMSHID | Issue Writer Certificate

Keywords

Acinetobacter baumannii

Biofilm

Antibiotic Resistance

Abstract

Background: Acinetobacter baumannii is capable of forming bioﬁ lms that may be responsible for the survival of this pathogen in the hospital environment as well as Antibiotic Resistance. Objectives: In this study, considering the importance of genes bap, blaPER-1, and csuE in the formation of bioﬁ lms and resistance to antimicrobial drugs, we aimed to investigate the frequency of these genes and also the relationship between these genes and the bioﬁ lm formation. Methods: One hundred and eighteen clinical strains of the A. baumannii were collected and identiﬁ ed using standard microbiological methods. Antibiotic susceptibility was evaluated by microdilution broth and disk diﬀ usion methods according to the Clinical and Laboratory Standards Institute (CLSI). Bioﬁ lm formation assay was performed by microtiter plate method. Then the bap, blaPER1, and csuE genes were detected by PCR. Results: The rate of XDR and MDR were 16. 1% and 83. 9%, respectively. Moreover, 9 (7. 6%) isolates were resistant to colistin. The results of bioﬁ lm formation revealed that 32 (27. 1%), 33 (28. 0%), 37 (31. 4%), and 16 (13. 6%) of the isolates had non-bioﬁ lm, weak, moderate, and strong activities, respectively. The association between the formation of bioﬁ lm and amikacin resistance was found (P < 0. 05). In the isolates, the frequencies of bap, blaPER-1, and csuE genes were 70. 3%, 54. 2%, and 93. 2%, respectively. Statistical analysis showed a signiﬁ cant correlation between the frequency of blaPER-1 and bap genes and the ability to form bioﬁ lms (P < 0. 05). Conclusions: This study shows the high tendency among the clinical isolates of A. baumannii to form a bioﬁ lm. It also shows the correlation between the presence of blaPER-1 and bap genes with the capacity of bioﬁ lm formation. Moreover, the majority (92. 4%) of the A. baumannii isolates from Isfahan were susceptible to colistin. Therefore, providing new and eﬀ ective strategies is essential for the prevention and treatment of infections caused by bioﬁ lm-forming A. baumannii strains.

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APA: Copy

Mahmoudi Monfared, Ahad, REZAEI, ALIAKBAR, POURSINA, FARKHONDEH, & FAGHRI, JAMSHID. (2019). Detection of Genes Involved in Bioﬁ lm Formation in MDR and XDR Acinetobacter baumannii Isolated from Human Clinical Specimens in Isfahan, Iran. ARCHIVES OF CLINICAL INFECTIOUS DISEASES, 14(2), 0-0. SID. https://sid.ir/paper/753800/en

Vancouver: Copy

Mahmoudi Monfared Ahad, REZAEI ALIAKBAR, POURSINA FARKHONDEH, FAGHRI JAMSHID. Detection of Genes Involved in Bioﬁ lm Formation in MDR and XDR Acinetobacter baumannii Isolated from Human Clinical Specimens in Isfahan, Iran. ARCHIVES OF CLINICAL INFECTIOUS DISEASES[Internet]. 2019;14(2):0-0. Available from: https://sid.ir/paper/753800/en

IEEE: Copy

Ahad Mahmoudi Monfared, ALIAKBAR REZAEI, FARKHONDEH POURSINA, and JAMSHID FAGHRI, “Detection of Genes Involved in Bioﬁ lm Formation in MDR and XDR Acinetobacter baumannii Isolated from Human Clinical Specimens in Isfahan, Iran,” ARCHIVES OF CLINICAL INFECTIOUS DISEASES, vol. 14, no. 2, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/753800/en

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