Snail-1 Silencing by siRNA Inhibits Migration of TE-8 Esophageal Cancer Cells Through Downregulation of Metastasis-Related Genes

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Hemmatzadeh Maryam; MOHAMMADI HAMED; Babaie Farhad; YOUSEFI MEHDI; Ebrazeh Mehrdad; MANSOORI BEHZAD; SHANEHBANDI DARIUSH; BARADARAN BEHZAD

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Journal: Advanced Pharmaceutical Bulletin Year:2018 | Volume:8 | Issue:3 Page(s): 437-445

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Title

Snail-1 Silencing by siRNA Inhibits Migration of TE-8 Esophageal Cancer Cells Through Downregulation of Metastasis-Related Genes

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Keywords

Esophageal cancer

Snail-1

siRNA

Apoptosis

Metastasis

Abstract

Purpose: Snail-1 is a transcription factor, which takes part in EMT, a process related to the emergence of invasion and cancer progression. The purpose of this study was to evaluate the effect of Snail-1 silencing on the human esophageal squamous cell carcinoma cell line, namely TE-8, in vitro. Methods: In this study, transfection of Snail-1 specific siRNA was conducted into TE-8 cells. The relative mRNA expression levels of Snail-1, Vimentin, CXCR4 and MMP-9 and transcription levels of miR-34a and let-7a were investigated by quantitative Real-time PCR. Western blotting was carried out to evaluate the Snail-1 protein level. Migration assay of TE-8 cells was also performed following the presence or absence of Snail-1 specific siRNA. MTT and TUNEL assays were performed to evaluate cell viability after Snail-1 silencing. Results: It was found that treatment of cancer cells with the Snail-specific siRNA effectively downregulated the expression of Snail-1 in both mRNA and protein levels, and vimentin, CXCR4, and MMP-9 in mRNA level. However, it elevated the transcript levels of miR-34a and let-7a expressions. Furthermore, transfection of cancer cells with the Snailspecific siRNA significantly induced Apoptosis in TE8 cells. Moreover, suppression of Snail-1 led to diminished cell migration. Conclusion: It seems that Snail-specific siRNA can significantly interrupt Esophageal cancer cell migration and reduce metastatic-related factors and induce miR-34a and let-7a in vitro. The bottom line is that therapeutic approaches via targeting Snail-1 can be used for ESCC treatment, suggesting that other possible target molecules for ESCC therapy require to be explored.

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APA: Copy

Hemmatzadeh, Maryam, MOHAMMADI, HAMED, Babaie, Farhad, YOUSEFI, MEHDI, Ebrazeh, Mehrdad, MANSOORI, BEHZAD, SHANEHBANDI, DARIUSH, & BARADARAN, BEHZAD. (2018). Snail-1 Silencing by siRNA Inhibits Migration of TE-8 Esophageal Cancer Cells Through Downregulation of Metastasis-Related Genes. ADVANCED PHARMACEUTICAL BULLETIN, 8(3), 437-445. SID. https://sid.ir/paper/753857/en

Vancouver: Copy

Hemmatzadeh Maryam, MOHAMMADI HAMED, Babaie Farhad, YOUSEFI MEHDI, Ebrazeh Mehrdad, MANSOORI BEHZAD, SHANEHBANDI DARIUSH, BARADARAN BEHZAD. Snail-1 Silencing by siRNA Inhibits Migration of TE-8 Esophageal Cancer Cells Through Downregulation of Metastasis-Related Genes. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2018;8(3):437-445. Available from: https://sid.ir/paper/753857/en

IEEE: Copy

Maryam Hemmatzadeh, HAMED MOHAMMADI, Farhad Babaie, MEHDI YOUSEFI, Mehrdad Ebrazeh, BEHZAD MANSOORI, DARIUSH SHANEHBANDI, and BEHZAD BARADARAN, “Snail-1 Silencing by siRNA Inhibits Migration of TE-8 Esophageal Cancer Cells Through Downregulation of Metastasis-Related Genes,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 8, no. 3, pp. 437–445, 2018, [Online]. Available: https://sid.ir/paper/753857/en

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