مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

154
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

66
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Expression of miR-664-3p in Osteosarcoma and Its Effects on the Proliferation and Apoptosis of Osteosarcoma Cells

Pages

  1817-1826

Abstract

 Background: To explore the expression level of miR-664-3p in Osteosarcoma and its effects on the Proliferation and Apoptosis of Osteosarcoma cells. Methods: Specimens of Osteosarcoma tissues were collected from 41 cases undergoing surgical treatment in the Orthopedics Department of Wuhan Puai Hospital, Wuhan, China from January 2015 to February 2018. Another 40 cases of normal bone tissue were collected. The expression of miR-664-3p were detected using quantificational real-time polymerase chain reaction. miR-664-3p mimics, miR-664-3p inhibitor and miR-6643p negative control (NC) were used to transfect U2-OS, which were named as mimics group, inhibitor group and NC group, respectively. MTT assay was adopted to detect the effects of microRNA-664-3p on the Proliferation of U2-OS after 24, 48, 72, 96 and 120 hours of transfection. Flow cytometry was applied to measure the Apoptosis rate of U2-OS after miR-664-3p transfection. Finally, Western Blot was employed to detect the expression of proteolipid protein 2 (PLP2). Results: The total Apoptosis rate of cells in the inhibitor group was obviously higher than those in the mimics group and the NC group (P<0. 001). The relative expression level of PLP2 in the inhibitor group was significantly lower than those in the mimics group and the NC group (P<0. 001). Conclusion: miR-664-3p may be involved in the occurrence and development of Osteosarcoma, and can regulate the Proliferation and Apoptosis of U2-OS cells, and the expression of PLP2. Besides, miR-664-3p may become a novel molecular biological indicator for the diagnosis, targeted treatment and prognosis assessment of Osteosarcoma.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    LI, Ye, TANG, JIE, HU, Yong, PENG, Yonghai, & WANG, Junwen. (2019). Expression of miR-664-3p in Osteosarcoma and Its Effects on the Proliferation and Apoptosis of Osteosarcoma Cells. IRANIAN JOURNAL OF PUBLIC HEALTH, 48(10), 1817-1826. SID. https://sid.ir/paper/754991/en

    Vancouver: Copy

    LI Ye, TANG JIE, HU Yong, PENG Yonghai, WANG Junwen. Expression of miR-664-3p in Osteosarcoma and Its Effects on the Proliferation and Apoptosis of Osteosarcoma Cells. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2019;48(10):1817-1826. Available from: https://sid.ir/paper/754991/en

    IEEE: Copy

    Ye LI, JIE TANG, Yong HU, Yonghai PENG, and Junwen WANG, “Expression of miR-664-3p in Osteosarcoma and Its Effects on the Proliferation and Apoptosis of Osteosarcoma Cells,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 48, no. 10, pp. 1817–1826, 2019, [Online]. Available: https://sid.ir/paper/754991/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button