NDRG2 Regulates the Expression of Genes Involved in Epithelial Mesenchymal Transition of Prostate Cancer Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Moradi Monfared Mohammad; Alizadeh Zarei Marziyeh; RAFIEI DEHBIDI GHOLAMREZA; BEHZAD BEHBAHANI ABBAS; ARABSOLGHAR RITA; TAKHSHID MOHAMMAD ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Medical Sciences Year:2019 | Volume:44 | Issue:2 Page(s): 118-126

Download Full-Text

View:

112

Download:

Cites:

Information Journal Paper

Title

NDRG2 Regulates the Expression of Genes Involved in Epithelial Mesenchymal Transition of Prostate Cancer Cells

Author(s)

Keywords

NDRG2 protein

human

Prostatic neoplasms

Cadherins

Vascular endothelial growth factors

Abstract

Background: Metastasis is the main cause of prostate cancer (PCa) death. The inhibitory effect of N-myc downstreamregulated gene 2 (NDRG2) on the invasiveness properties of PCa cells has been demonstrated previously. However, its underlying mechanisms have not yet been investigated. The present study aimed to investigate the effects of NDRG2 overexpression on the expression of genes involved in epithelial-mesenchymal transition (EMT) including E-cadherin (E-CAD), α-and β-catenins, Slug and Snail, transforming growth factor (TGF)-α and-β , and vascular endothelial growth factor (VEGF). Methods: In the present in vitro study, LNCaP cells were divided into three groups, namely NDRG2 group (transfected with PSES-pAdenoVator-PSA-NDRG2-IRES-GFP plasmid), mock group (transfected with mock plasmid), and control group (without transfection). The effect of NDRG2 overexpression on the migration and invasion of LNCaP cells were investigated using the transwell assay. Real-time PCR was used for the evaluation of gene expression. For the statistical analyses, one-way ANOVA, student t test or Mann-Whitney U test were applied using the SPSS software (version 15. 0). P values <0. 05 were considered statistically significant. Results: The results demonstrated that the overexpression of NDRG2 reduced the invasion and migration of LNCaP cells compared to the control and mock groups (P<0. 001). A decreased expression of TGF-β (P=0. 002), VEGF (P=0. 014), Slug (P=0. 005), and Snail (P=0. 012); and an increased expression of E-CAD (P=0. 009) were observed following NDRG2 overexpression in LNCaP cells. Conclusion: The results of the present study suggest that NDRG2 inhibits the invasiveness properties of LNCaP cells probably through changes in the expression of genes involved in EMT.

Cites

No record.

References

No record.

Cite

APA: Copy

Moradi Monfared, Mohammad, Alizadeh Zarei, Marziyeh, RAFIEI DEHBIDI, GHOLAMREZA, BEHZAD BEHBAHANI, ABBAS, ARABSOLGHAR, RITA, & TAKHSHID, MOHAMMAD ALI. (2019). NDRG2 Regulates the Expression of Genes Involved in Epithelial Mesenchymal Transition of Prostate Cancer Cells. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 44(2), 118-126. SID. https://sid.ir/paper/755323/en

Vancouver: Copy

Moradi Monfared Mohammad, Alizadeh Zarei Marziyeh, RAFIEI DEHBIDI GHOLAMREZA, BEHZAD BEHBAHANI ABBAS, ARABSOLGHAR RITA, TAKHSHID MOHAMMAD ALI. NDRG2 Regulates the Expression of Genes Involved in Epithelial Mesenchymal Transition of Prostate Cancer Cells. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2019;44(2):118-126. Available from: https://sid.ir/paper/755323/en

IEEE: Copy

Mohammad Moradi Monfared, Marziyeh Alizadeh Zarei, GHOLAMREZA RAFIEI DEHBIDI, ABBAS BEHZAD BEHBAHANI, RITA ARABSOLGHAR, and MOHAMMAD ALI TAKHSHID, “NDRG2 Regulates the Expression of Genes Involved in Epithelial Mesenchymal Transition of Prostate Cancer Cells,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 44, no. 2, pp. 118–126, 2019, [Online]. Available: https://sid.ir/paper/755323/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops