Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine

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SOLTANPOUR GHARIBDOUSTI FAEZEH; FAZELI DELSHAD BANAFSHEH; FALAK REZA; SHAYANFAR NASRIN; GANJALIKHANI HAKEMI MAZDAK; ANDALIB ALIREZA; KARDAR GHOLAM ALI

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Journal: Iranian Journal of Basic Medical Sciences Year:2020 | Volume:23 | Issue:4 Page(s): 507-514

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Title

Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine

Author(s)

Keywords

Angiogenesis

Immunoinformatics

Metastasis

Peptide-based vaccine

VEGF

Abstract

Objective(s): Blocking of vascular endothelial growth factor (VEGF) plays a pivotal role in inhibition of Metastasis and is a target for development of anti-angiogenic agents. In this study, a Peptide-based vaccine was designed and its potential for induction of humoral immune responses, generation of neutralizing antibodies, inhibition of tumor growth and Metastasis was determined. Materials and Methods: With online bioinformatics tools, a fragment of the VEGF-A was selected for a Peptide-based vaccine. To enhance its antigenicity, the peptide was conjugated with Keyhole limpet hemocyanin and used to immunize mice. Then, the polyclonal anti-VEGF antibody titer was measured and its effect on proliferation of HUVEC cell line was investigated by MTT assay. Finally, we checked the effect of the peptide on tumor growth, Metastasis, and survival rates in a mouse model of cancer. Results: The bioinformatics analysis of the selected region confirmed dis-similarity of the peptide with any other human protein and its acceptable antigenicity to stimulate a tumor-specific humoral response. Anti-VEGF antibody titers were significantly greater in vaccinated mice than in controls. IgG antibody from mice immunized with recombinant VEGF-A inhibited HUVEC proliferation (P<0. 0001). Tumors in vaccinated mice were significantly smaller than those in controls. Moreover, Metastasis was reduced and survival rates increased in the vaccinated group. Conclusion: Production of high-titer antibody against the peptide vaccine indicated that the peptide has the potency to be used as a Peptide-based vaccine for humoral inhibition of tumor growth and Metastasis. The efficacy of the peptide should be further tested in primate models.

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APA: Copy

SOLTANPOUR GHARIBDOUSTI, FAEZEH, FAZELI DELSHAD, BANAFSHEH, FALAK, REZA, SHAYANFAR, NASRIN, GANJALIKHANI HAKEMI, MAZDAK, ANDALIB, ALIREZA, & KARDAR, GHOLAM ALI. (2020). Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(4), 507-514. SID. https://sid.ir/paper/756164/en

Vancouver: Copy

SOLTANPOUR GHARIBDOUSTI FAEZEH, FAZELI DELSHAD BANAFSHEH, FALAK REZA, SHAYANFAR NASRIN, GANJALIKHANI HAKEMI MAZDAK, ANDALIB ALIREZA, KARDAR GHOLAM ALI. Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(4):507-514. Available from: https://sid.ir/paper/756164/en

IEEE: Copy

FAEZEH SOLTANPOUR GHARIBDOUSTI, BANAFSHEH FAZELI DELSHAD, REZA FALAK, NASRIN SHAYANFAR, MAZDAK GANJALIKHANI HAKEMI, ALIREZA ANDALIB, and GHOLAM ALI KARDAR, “Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 4, pp. 507–514, 2020, [Online]. Available: https://sid.ir/paper/756164/en

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