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Information Journal Paper

Title

The Effect of Beta-Boswellic Acid on the Expression of Camk4 and Camk2α Genes in the PC12 Cell Line

Pages

  437-443

Abstract

 Purpose: Beta-boswellic acid (β BA) may play central roles in neural plasticity. Neural plasticity has significant implications for learning and Memory which are governed by strict Memoryrelated molecular pathways. To gain insight into the molecular mechanism by which β BA affects these pathways this study analyzed the expression patterns of Camk2α and Camk4 genes in PC12 cells treated with β BA. Methods: The cytotoxic effects of different β BA concentrations on PC12 cells were examined by MTT assay. For gene expression analysis, cells were treated with concentrations of 1 and 10 μ M of β BA for 12, 24, 48, and 72 hours. Total RNA was purified by RNX-Plus solution and reverse transcribed into cDNA using Thermo Scientific Reverse Transcription reagents. The expression patterns of Camk2α and Camk4 genes were quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: MTT assay indicated that β BA reduced PC12 cell viability in a time-and concentrationdependent manner. The 50% inhibitory concentrations for the 48 and 72 hours time points were 35 and 26 μ M, respectively; while, the β BA concentrations up to 100 μ M failed to kill 50% of the cells after 24 hours. According to the qRT-PCR data, the Camk2α variant is not expressed in either β BA-treated or untreated PC12 cells. However, a significant upregulation was observed in Camk4 after 12 hours of treatment with β BA, which followed by a significant downregulation after 24 hours and a persistent expression equal to the control until 72 hours. Conclusion: these findings indicate that PC12 cells not only does not express Camk2α but also its expression cannot be induced by β BA. However, β BA does modulate the expression of Camk4. This result provides further insight into the molecular mechanism by which β BA affects Memory.

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    APA: Copy

    Jebelli, Asiyeh, KHALAJ KONDORI, MOHAMMAD, & RAHMATI YAMCHI, MOHAMMAD. (2020). The Effect of Beta-Boswellic Acid on the Expression of Camk4 and Camk2α Genes in the PC12 Cell Line. ADVANCED PHARMACEUTICAL BULLETIN, 10(3), 437-443. SID. https://sid.ir/paper/756760/en

    Vancouver: Copy

    Jebelli Asiyeh, KHALAJ KONDORI MOHAMMAD, RAHMATI YAMCHI MOHAMMAD. The Effect of Beta-Boswellic Acid on the Expression of Camk4 and Camk2α Genes in the PC12 Cell Line. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2020;10(3):437-443. Available from: https://sid.ir/paper/756760/en

    IEEE: Copy

    Asiyeh Jebelli, MOHAMMAD KHALAJ KONDORI, and MOHAMMAD RAHMATI YAMCHI, “The Effect of Beta-Boswellic Acid on the Expression of Camk4 and Camk2α Genes in the PC12 Cell Line,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 10, no. 3, pp. 437–443, 2020, [Online]. Available: https://sid.ir/paper/756760/en

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