Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells (SHORT COMMUNICATION)

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MOHAMMADI FARSANI TAIEBEH; MOTEVASELI ELAHE; NEYAZI NADIA; KHORRAMIZADEH MOHAMMAD REZA; Zafarvahedian Elaheh; GHAHREMANI MOHAMMAD HOSSEIN

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:1 Page(s): 70-75

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Title

Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells (SHORT COMMUNICATION)

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Keywords

Metalloendopeptidase

Alzheimer's disease

Caco-2 cells

Abstract

Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cell line (Caco-2). Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity were assessed by Western blot analysis and fluorescent assay, respectively. Results: Our results confirmed that the amount of IDE was higher in cell extract compared to supernatant, and different passage numbers and culture times had small effect on IDE expression. However, when cells were cultured in the passage number range of 5-15 for 72 hours, the IDE activity was 35% higher compared to other passage numbers (p < 0. 05). Conclusion: The use of Caco-2 cells at passage number range of 5-15 and culture time of 72 hours provides proper conditions for IDE-related studies.

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APA: Copy

MOHAMMADI FARSANI, TAIEBEH, MOTEVASELI, ELAHE, NEYAZI, NADIA, KHORRAMIZADEH, MOHAMMAD REZA, & Zafarvahedian, Elaheh. (2018). Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells (SHORT COMMUNICATION). IRANIAN BIOMEDICAL JOURNAL, 22(1), 70-75. SID. https://sid.ir/paper/759083/en

Vancouver: Copy

MOHAMMADI FARSANI TAIEBEH, MOTEVASELI ELAHE, NEYAZI NADIA, KHORRAMIZADEH MOHAMMAD REZA, Zafarvahedian Elaheh. Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells (SHORT COMMUNICATION). IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(1):70-75. Available from: https://sid.ir/paper/759083/en

IEEE: Copy

TAIEBEH MOHAMMADI FARSANI, ELAHE MOTEVASELI, NADIA NEYAZI, MOHAMMAD REZA KHORRAMIZADEH, and Elaheh Zafarvahedian, “Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells (SHORT COMMUNICATION),” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 1, pp. 70–75, 2018, [Online]. Available: https://sid.ir/paper/759083/en

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