Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line

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TORKASHVAND FATEMEH; MAHBOUDI FEREIDOUN; Vossoughi Manouchehr; Fatemi Elnaz; Moosavi Basri Seyed Masoud; HEYDARI AMIR; VAZIRI BEHROUZ

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:6 Page(s): 385-393

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Title

Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line

Author(s)

Keywords

CHO cells

Glutathione

Monoclonal antibodies

Proteomics

Abstract

Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated. Results: The Glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group. Conclusion: Our findings could be helpful to identify new targets for metabolic engineering.

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APA: Copy

TORKASHVAND, FATEMEH, MAHBOUDI, FEREIDOUN, Vossoughi, Manouchehr, Fatemi, Elnaz, Moosavi Basri, Seyed Masoud, HEYDARI, AMIR, & VAZIRI, BEHROUZ. (2018). Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line. IRANIAN BIOMEDICAL JOURNAL, 22(6), 385-393. SID. https://sid.ir/paper/759169/en

Vancouver: Copy

TORKASHVAND FATEMEH, MAHBOUDI FEREIDOUN, Vossoughi Manouchehr, Fatemi Elnaz, Moosavi Basri Seyed Masoud, HEYDARI AMIR, VAZIRI BEHROUZ. Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(6):385-393. Available from: https://sid.ir/paper/759169/en

IEEE: Copy

FATEMEH TORKASHVAND, FEREIDOUN MAHBOUDI, Manouchehr Vossoughi, Elnaz Fatemi, Seyed Masoud Moosavi Basri, AMIR HEYDARI, and BEHROUZ VAZIRI, “Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 6, pp. 385–393, 2018, [Online]. Available: https://sid.ir/paper/759169/en

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