An Experimental Study on Spinal Cord μ-Opioid and α 2-Adrenergic Receptors mRNA Expression Following Stress-Induced Hyperalgesia in Male Rats

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Hormoz Asef; Zarifkar Asadollah; Rostami Bahar; NAGHIBALHOSSAINI FAKHRADDIN

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Journal: Iranian Journal of Medical Sciences Year:2019 | Volume:44 | Issue:5 Page(s): 397-405

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Title

An Experimental Study on Spinal Cord μ-Opioid and α 2-Adrenergic Receptors mRNA Expression Following Stress-Induced Hyperalgesia in Male Rats

Author(s)

Hormoz Asef | Zarifkar Asadollah | Rostami Bahar | NAGHIBALHOSSAINI FAKHRADDIN | Issue Writer Certificate

Keywords

Stress

Hyperalgesia

Spinal cord

Receptors

opioid

Adrenergic alpha-2 receptor antagonists

Abstract

Background: Intense Stress can change pain perception and induce Hyperalgesia; a phenomenon called Stress-induced Hyperalgesia (SIH). However, the neurobiological mechanism of this effect remains unclear. The present study aimed to investigate the effect of the Spinal cord μ-opioid Receptors (MOR) and α 2-adrenergic Receptors (α 2-AR) on pain sensation in rats with SIH. Methods: Eighteen Sprague-Dawley male rats, weighing 200-250 g, were randomly divided into two groups (n=9 per group), namely the control and Stress group. The Stress group was evoked by random 1-hour daily foot-shock Stress (0. 8 mA for 10 seconds, 1 minute apart) for 3 weeks using a communication box. The tail-flick and formalin tests were performed in both groups on day 22. The real-time RT-PCR technique was used to observe MOR and α 2-AR mRNA levels at the L4-L5 lumbar Spinal cord. Statistical analysis was performed using the GraphPad Prism 5 software (San Diego, CA, USA). Student’ s t test was applied for comparisons between the groups. P<0. 05 was considered statistically significant. Results: There was a significant (P=0. 0014) decrease in tailflick latency in the Stress group compared to the control group. Nociceptive behavioral responses to formalin-induced pain in the Stress group were significantly increased in the acute (P=0. 007) and chronic (P=0. 001) phases of the formalin test compared to the control group. A significant reduction was also observed in MOR mRNA level of the Stress group compared to the control group (P=0. 003). There was no significant difference in α 2-AR mRNA level between the Stress and control group. Conclusion: The results indicate that chronic Stress can affect nociception and lead to Hyperalgesia. The data suggest that decreased expression of Spinal cord MOR causes Hyperalgesia.

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APA: Copy

Hormoz, Asef, Zarifkar, Asadollah, Rostami, Bahar, & NAGHIBALHOSSAINI, FAKHRADDIN. (2019). An Experimental Study on Spinal Cord μ-Opioid and α 2-Adrenergic Receptors mRNA Expression Following Stress-Induced Hyperalgesia in Male Rats. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 44(5), 397-405. SID. https://sid.ir/paper/759221/en

Vancouver: Copy

Hormoz Asef, Zarifkar Asadollah, Rostami Bahar, NAGHIBALHOSSAINI FAKHRADDIN. An Experimental Study on Spinal Cord μ-Opioid and α 2-Adrenergic Receptors mRNA Expression Following Stress-Induced Hyperalgesia in Male Rats. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2019;44(5):397-405. Available from: https://sid.ir/paper/759221/en

IEEE: Copy

Asef Hormoz, Asadollah Zarifkar, Bahar Rostami, and FAKHRADDIN NAGHIBALHOSSAINI, “An Experimental Study on Spinal Cord μ-Opioid and α 2-Adrenergic Receptors mRNA Expression Following Stress-Induced Hyperalgesia in Male Rats,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 44, no. 5, pp. 397–405, 2019, [Online]. Available: https://sid.ir/paper/759221/en

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