PRENATAL DIAGNOSIS OF HUMAN FETAL RH BLOOD GROUP BY HEMINESTED-PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHANGARI GHASEM; SEID MOHAMADPOOR M.SH.; SHAMS LAHIJANI M.; RABANI B.; MOHSENI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pediatrics Year:2004 | Volume:14 | Issue:1 Page(s): 5-14

Download Full-Text

Persian Verion

View:

6,437

Download:

Cites:

Information Journal Paper

Title

PRENATAL DIAGNOSIS OF HUMAN FETAL RH BLOOD GROUP BY HEMINESTED-PCR

Author(s)

Keywords

HEMOLYTIC DISEASE OF THE NEWBORN (HDN)Q4

RHD GENEQ4

HEMINESTED-PCRQ4

CHORIONIC VILLUS SAMPLING (CVS)Q4

Abstract

Background: The rhesus blood group antigen system is important in transfusion and clinical medicine, being involved in hemolytic disease of the newborn, transfusion reactions and autoimmune hemolytic anemia. Despite the widespread use of rhesus immunoglobulin prophylaxis in rhesus D (RhD)-negative mothers, rhesus immunization still occurs. Knowledge of the RhD status of the fetus is important in the clinical management, because no further diagnosis or therapeutic procedures are necessary if the fetus is RhD-negative. RhD antigen can be detected using a sensitive PCR-based assay. It was shown that RhD negative individuals lack the RHD GENE. Methods: We obtained 5ml blood samples from thirty eight RhD positive and negative blood donors, as controls and forty chorionic villus samples (CVS) from pregnant women at 8 to 12 weeks of gestation. DNA was extracted from CVS by standard salting out and blood DNA was extracted by boiling procedure. DNA amplification (heminested-PCR) was carried out with appropriate primers. Results: PCR products were analyzed on an agarose gel. RHD GENE determined in all CV samples.

Cites

No record.

References

No record.

Cite

APA: Copy

AHANGARI, GHASEM, SEID MOHAMADPOOR, M.SH., SHAMS LAHIJANI, M., RABANI, B., & MOHSENI, F.. (2004). PRENATAL DIAGNOSIS OF HUMAN FETAL RH BLOOD GROUP BY HEMINESTED-PCR. IRANIAN JOURNAL OF PEDIATRICS, 14(1), 5-14. SID. https://sid.ir/paper/75989/en

Vancouver: Copy

AHANGARI GHASEM, SEID MOHAMADPOOR M.SH., SHAMS LAHIJANI M., RABANI B., MOHSENI F.. PRENATAL DIAGNOSIS OF HUMAN FETAL RH BLOOD GROUP BY HEMINESTED-PCR. IRANIAN JOURNAL OF PEDIATRICS[Internet]. 2004;14(1):5-14. Available from: https://sid.ir/paper/75989/en

IEEE: Copy

GHASEM AHANGARI, M.SH. SEID MOHAMADPOOR, M. SHAMS LAHIJANI, B. RABANI, and F. MOHSENI, “PRENATAL DIAGNOSIS OF HUMAN FETAL RH BLOOD GROUP BY HEMINESTED-PCR,” IRANIAN JOURNAL OF PEDIATRICS, vol. 14, no. 1, pp. 5–14, 2004, [Online]. Available: https://sid.ir/paper/75989/en

Related Journal Papers