9-cis-Retinoic Acid and 1, 25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways

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Saeb Saeedeh; Azari Hassan; MOSTAFAVI POUR ZOHREH; GHANBARI AMIR; EBRAHIMI SEPIDEH; MOKARRAM POONEH

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Journal: IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS) Year:2018 | Volume:43 | Issue:5 Page(s): 523-532

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Title

9-cis-Retinoic Acid and 1, 25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways

Author(s)

Keywords

Cell differentiation

Oligodendrocyte precursor cells

9-cis-retinoic acid

25-dihydroxy vitamin D3

Receptors

Wnt

Notch signaling pathway

Abstract

Background: Differentiating Oligodendrocyte precursor cells (OPCs) into oligodendrocytes could be improved by inhibiting signaling pathways such as Wnt and Notch. 9-cis-retinoic acid (9-cis-RA) and 1, 25-dihydroxyvitamin D3 (1, 25[OH]2D3) can ameliorate oligodendrogenesis. We investigated whether they could increase oligodendrogenesis by inhibiting the Wnt and Notch signaling pathways. Methods: Cortical neural stem cells were isolated from 14-dayold rat embryos and cultured using the neurosphere assay. The cells were treated in 4 different conditions for 1 week: the negative control group received only the basic fibroblast growth factor, the positive control group received only T3 without growth factors, the RA group was treated with 9-cis-RA, and the Vit D3 group was treated with 1, 25(OH)2D3. The effects of 9-cis-RA and 1, 25(OH)2D3 on the level of the myelin basic protein (MBP) and the gene expression of the SOX10, MBP gene, HES5, and LRP6 were studied using flow cytometry and real-time PCR. The data were analyzed using one-way ANOVA with GraphPad Prism. A P value less than 0. 05 was considered significant. Results: The mRNA expressions of the SOX10, MBP, and MBP gene were significantly increased in the treated groups compared with the negative control group; the increase was similar in the 9-cis-RA group and the positive control group. Furthermore, 9-cis-RA significantly decreased the expression of the HES5 gene, a Notch signaling pathway transcription factor, and 1, 25(OH)2D3 significantly reduced the expression of the LRP6 gene, a Wnt signaling pathway co-receptor. Conclusion: It seems that 9-cis-RA and 1, 25(OH)2D3 are good candidates to improve the differentiation of OPCs into oligodendrocytes.

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APA: Copy

Saeb, Saeedeh, Azari, Hassan, MOSTAFAVI POUR, ZOHREH, GHANBARI, AMIR, EBRAHIMI, SEPIDEH, & MOKARRAM, POONEH. (2018). 9-cis-Retinoic Acid and 1, 25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 43(5), 523-532. SID. https://sid.ir/paper/761623/en

Vancouver: Copy

Saeb Saeedeh, Azari Hassan, MOSTAFAVI POUR ZOHREH, GHANBARI AMIR, EBRAHIMI SEPIDEH, MOKARRAM POONEH. 9-cis-Retinoic Acid and 1, 25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2018;43(5):523-532. Available from: https://sid.ir/paper/761623/en

IEEE: Copy

Saeedeh Saeb, Hassan Azari, ZOHREH MOSTAFAVI POUR, AMIR GHANBARI, SEPIDEH EBRAHIMI, and POONEH MOKARRAM, “9-cis-Retinoic Acid and 1, 25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 43, no. 5, pp. 523–532, 2018, [Online]. Available: https://sid.ir/paper/761623/en

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