مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

191
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

129
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Design and Microfabrication of An On-Chip Oocyte Maturation System for Reduction of Apoptosis

Pages

  32-39

Abstract

 Objective: In customary Assisted Reproductive Technology (ART), Oocyte culture occurs in static micro drops of Petri dishes with vast media volume; while, the in vivo condition is dynamic. In this study, we aimed to improve the maturation efficiency of mammalian Oocytes by designing an optimal microchamber array to obtain the integration of Oocyte trapping and maturation within a microfluidic device and evaluate the role of microfluidic culture condition in lipid peroxidation level of the culture medium, in vitro matured Oocytes Apoptosis, and its comparison with the conventional static system. Materials and Methods: In this experimental research, immature Oocytes were collected from ovaries of the Naval Medical Research Institute (NMRI) mice. Oocytes were randomly laid in static and dynamic (passive & active) In vitro maturation culture medium for 24 hours. The lipid peroxidation level in Oocyte culture media was assessed by measuring the concentration of malondialdehyde (MDA), and the rate of Apoptosis in in vitro matured Oocytes was assessed by the TUNEL assay after a-24 hour maturation period. Results: The MDA concentration in both dynamic Oocyte maturation media were significantly lower than the static medium (0. 003 and 0. 002 vs. 0. 13 μ mol/L, P<0. 01). Moreover, the rate of Apoptosis in matured Oocytes after a-24 hour maturation period was significantly lower in passive dynamic and active dynamic groups compared with the static group (16%, 15% vs. 35%, P<0. 01). Conclusions: The dynamic culture for in vitro Oocyte maturation (IVM) improves the viability of IVM Oocytes in comparison with the static culture condition.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    SADEGHZADEH OSKOUEI, BEHNAZ, Zargari, Siavash, SHAHABI, PARVIZ, GHAFFARI NOVIN, MAREFAT, & Pashaiasl, Maryam. (2021). Design and Microfabrication of An On-Chip Oocyte Maturation System for Reduction of Apoptosis. CELL JOURNAL (YAKHTEH), 23(1 (89)), 32-39. SID. https://sid.ir/paper/762720/en

    Vancouver: Copy

    SADEGHZADEH OSKOUEI BEHNAZ, Zargari Siavash, SHAHABI PARVIZ, GHAFFARI NOVIN MAREFAT, Pashaiasl Maryam. Design and Microfabrication of An On-Chip Oocyte Maturation System for Reduction of Apoptosis. CELL JOURNAL (YAKHTEH)[Internet]. 2021;23(1 (89)):32-39. Available from: https://sid.ir/paper/762720/en

    IEEE: Copy

    BEHNAZ SADEGHZADEH OSKOUEI, Siavash Zargari, PARVIZ SHAHABI, MAREFAT GHAFFARI NOVIN, and Maryam Pashaiasl, “Design and Microfabrication of An On-Chip Oocyte Maturation System for Reduction of Apoptosis,” CELL JOURNAL (YAKHTEH), vol. 23, no. 1 (89), pp. 32–39, 2021, [Online]. Available: https://sid.ir/paper/762720/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button