EXPRESSION OF RECOMBINANT HC DOMAIN OF CLOSTRIDIUM BOTULINUM NEUROTOXIN A IN E. COLI AND ITS PURIFICATION AS A VACCINE CANDIDATE AGAINST BOTULISM

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FATEMI S.S.A.; YARI KH.; TAVALLAEI M.; KAHRIZI D.

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Paper Information

Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2011 | Volume:16 | Issue:2 (SERIAL NUMBER 60) Page(s): 36-44

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Title

EXPRESSION OF RECOMBINANT HC DOMAIN OF CLOSTRIDIUM BOTULINUM NEUROTOXIN A IN E. COLI AND ITS PURIFICATION AS A VACCINE CANDIDATE AGAINST BOTULISM

Author(s)

FATEMI S.S.A. | YARI KH. | TAVALLAEI M. | KAHRIZI D. | Issue Writer Certificate

Keywords

PROTEIN EXPRESIONQ2

E. COLIQ2

CLOSTRIDIUM BOTULINUMQ3

BOTULISMQ3

Abstract

Background and Aim: BOTULISM is a lethal disease which is caused by the one of the neurotoxins of the 7 types of CLOSTRIDIUM BOTULINUM. The carboxylic domain of the heavy chain of CLOSTRIDIUM BOTULINUM type A neurotoxin (BoNT/A-Hc), is highly capable of activating immune system and is used for production of a vaccine against BOTULISM. The aim of this study was to express and produce soluble form of BoNT/A-Hc in recombinant E. COLI.Materials and Methods: Hc part of BoNT/A was subcloned in pET28a vector and clone was expressed in E. COLI BL21 (DE3). Then the best recombinant clones were selected based on three main factors: expression, growth and plasmid stability. Then protein expression was evaluated in LB and M9 media and the protein was purified by resin column chromatography.Results: In flask culture, under optimal conditions of bacterial culture yielded 52mg of BoNT/A-Hc soluble protein from each liter of culture medium.Conclusions: According to the results of this study, selection of suitable strains on the basis of important growth indices of the bacteria, expression of recombinant protein and plasmid stability can lead to in increased efficiency of the recombinant protein production.

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APA: Copy

FATEMI, S.S.A., YARI, KH., TAVALLAEI, M., & KAHRIZI, D.. (2011). EXPRESSION OF RECOMBINANT HC DOMAIN OF CLOSTRIDIUM BOTULINUM NEUROTOXIN A IN E. COLI AND ITS PURIFICATION AS A VACCINE CANDIDATE AGAINST BOTULISM. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 16(2 (SERIAL NUMBER 60)), 36-44. SID. https://sid.ir/paper/76360/en

Vancouver: Copy

FATEMI S.S.A., YARI KH., TAVALLAEI M., KAHRIZI D.. EXPRESSION OF RECOMBINANT HC DOMAIN OF CLOSTRIDIUM BOTULINUM NEUROTOXIN A IN E. COLI AND ITS PURIFICATION AS A VACCINE CANDIDATE AGAINST BOTULISM. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;16(2 (SERIAL NUMBER 60)):36-44. Available from: https://sid.ir/paper/76360/en

IEEE: Copy

S.S.A. FATEMI, KH. YARI, M. TAVALLAEI, and D. KAHRIZI, “EXPRESSION OF RECOMBINANT HC DOMAIN OF CLOSTRIDIUM BOTULINUM NEUROTOXIN A IN E. COLI AND ITS PURIFICATION AS A VACCINE CANDIDATE AGAINST BOTULISM,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 16, no. 2 (SERIAL NUMBER 60), pp. 36–44, 2011, [Online]. Available: https://sid.ir/paper/76360/en

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