ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF KURDISTAN PROPOLIS ON STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS AND PSEUDOMONAS AERUGINOSA

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Khosravi Neshat; DARVISHI SHOALEH; DAVARI KAMBIZ

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Journal: SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:20 | Issue:6 Page(s): 97-106

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Title

ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF KURDISTAN PROPOLIS ON STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS AND PSEUDOMONAS AERUGINOSA

Author(s)

Khosravi Neshat | DARVISHI SHOALEH | DAVARI KAMBIZ | Issue Writer Certificate

Keywords

PROPOLISQ2

INHIBITIONQ2

ANTIQ2

BACTERIAQ2

STAPHYLOCOCCUS AUREUSQ2

BACILLUS CEREUSQ2

PSEUDOMONAS AERUGINOSAQ2

Abstract

Background and Aim: STAPHYLOCOCCUS AUREUS, PSEUDOMONAS AERUGINOSA, BACILLUS CEREUS can cause food poisoning and digestive disorders in human beings. PROPOLIS has been known as an ANTImicrobial agent against BACTERIA. The aim of this study was to determine the in-vitro ANTImicrobial activity of PROPOLIS extract on BACTERIAl strains.Material and Methods: In this study, we evaluated ANTIbacterial effects of PROPOLIS alcoholic extract on STAPHYLOCOCCUS AUREUS (ptcc1112), BACILLUS CEREUS (ptcc1247) and PSEUDOMONAS AERUGINOSA (ptcc1707) for three times, by means of paper disc method (determination of INHIBITION zone diameter) and broth dilution, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Using SPSS software, statistical analysis was performed by DUNCAN test.Results: The results showed that the ANTIbacterial activity and INHIBITION diameters for STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS and PSEUDOMONAS AERUGINOSA (in a PROPOLIS extract concentration of 5.25 mg / ml) were respectively 18.66±0.58, 18, 14.33±0.58, respectively. S. Aureus had the highest sensitivity and PSEUDOMONAS AERUGINOSA showed the highest resistance to alcoholic extract of PROPOLIS. The minimum inhibitory concentrations (MIC) for STAPHYLOCOCCUS AUREUS, PSEUDOMONAS AERUGINOSA and BACILLUS CEREUS were 0.656, 0.656 and 2.62 mg/ ml respectively and the lethal concentrations (MBC) for Clostridium sporogenes, Escherichia coli and STAPHYLOCOCCUS AUREUS, were 0.656, 0.656 and 2.62 mg/ ml respectively.Conclusion: The alcoholic extract of PROPOLIS had inhibitory and ANTIbacterial effects on STAPHYLOCOCCUS AUREUS, PSEUDOMONAS AERUGINOSA and BACILLUS CEREUS. Considering organoleptic effects of PROPOLIS extract, it can be used as a preservative in food.

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APA: Copy

Khosravi, Neshat, DARVISHI, SHOALEH, & DAVARI, KAMBIZ. (2016). ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF KURDISTAN PROPOLIS ON STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS AND PSEUDOMONAS AERUGINOSA. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 20(6), 97-106. SID. https://sid.ir/paper/76467/en

Vancouver: Copy

Khosravi Neshat, DARVISHI SHOALEH, DAVARI KAMBIZ. ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF KURDISTAN PROPOLIS ON STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS AND PSEUDOMONAS AERUGINOSA. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;20(6):97-106. Available from: https://sid.ir/paper/76467/en

IEEE: Copy

Neshat Khosravi, SHOALEH DARVISHI, and KAMBIZ DAVARI, “ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF KURDISTAN PROPOLIS ON STAPHYLOCOCCUS AUREUS, BACILLUS CEREUS AND PSEUDOMONAS AERUGINOSA,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 20, no. 6, pp. 97–106, 2016, [Online]. Available: https://sid.ir/paper/76467/en

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