Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies

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HASHEMI ZAHRA SADAT; FOROUZANDEH MOGHADAM MEHDI; SADRODDINY ESMAEIL

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Journal: International Journal of Cancer Management Year:2018 | Volume:11 | Issue:8 Page(s): 0-0

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Title

Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies

Author(s)

HASHEMI ZAHRA SADAT | FOROUZANDEH MOGHADAM MEHDI | SADRODDINY ESMAEIL | Issue Writer Certificate

Keywords

miR-193-3p

Metastasis

Breast Cancer

Abstract

Background: Metastasis-associated miRNA (metastamiR) harbors a great potential to confine Metastasis as the most lethal aspect of cancer. miR-193b-3p is an anti-metastatic miRNA, whose expression significantly decreases in metastatic Breast Cancer cells. Objectives: In the present study, the expression patterns of different cell-lines were investigated, following an effective miR transfection strategy. Methods: Double-stranded oligo of mature miR-193b-3p, miR-negative, and miR-LacZ were designed and cloned into pcDNA6. 2gw/EmGFP plasmid. Calculating the population doubling time (PDT), non-tumorigenic MCF-10A, tumorigenic but non-metastatic MCF-7, and metastatic MDA-MB231 were transfected by Lipofectamin2000 and Express-In. The expressions of miR-193b-3p and miR-191 have been quantified by Real-time PCR 48 hours after transfection. Scratch, Transwell migration, and Matrigel invasion assays have been carried out to assess the migration and invasion levels of the cell-lines. Results: The PDT (21. 27 ± 0. 43, 28. 18 ± 0. 34, and 35. 83 ± 0. 44 hours) and miR-193b-3p relative expression before transfection (100, 42 and 8) were measured for MCF-10A, MCF-7, and MDA-MB231 cell-lines, respectively. Better transfection results were obtained based on nano-liposome method. The expression of the miR-193b-3p was increased 32, 19 and, 65 fold-change. The rate of invasion in metastatic cells was 6. 6 fold-higher in comparison to MCF-7 cells. Conclusions: Higher expression rate of the miRNA is anticipated to occur, following miR-mimic transfection. However, the observed differential patterns of miRNA increase in the context of different cell-lines, indicating the involvement of more complicated cellular pathways. Scrutinizing these cellular mechanisms could open new horizons in cancer therapy and management strategies.

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APA: Copy

HASHEMI, ZAHRA SADAT, FOROUZANDEH MOGHADAM, MEHDI, & SADRODDINY, ESMAEIL. (2018). Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 11(8), 0-0. SID. https://sid.ir/paper/765188/en

Vancouver: Copy

HASHEMI ZAHRA SADAT, FOROUZANDEH MOGHADAM MEHDI, SADRODDINY ESMAEIL. Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2018;11(8):0-0. Available from: https://sid.ir/paper/765188/en

IEEE: Copy

ZAHRA SADAT HASHEMI, MEHDI FOROUZANDEH MOGHADAM, and ESMAEIL SADRODDINY, “Varying miR-193b-3p Expression Patterns in Breast Cancer Cell Lines Indicates Its Potential for Cancer Management Strategies,” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 11, no. 8, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/765188/en

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